Parasitology Research

, Volume 102, Issue 1, pp 171–174 | Cite as

Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Liston, Anopheles culicifacies, species C) and filaria vector (Culex quinquefasciatus Say)

  • Vibha Pandey
  • Veena Agrawal
  • K. Raghavendra
  • A. P. Dash
Short Communication

Abstract

A system for biocontrol of malaria and filarial mosquito vectors has been developed using herbal extracts of three Spilanthes species, S. acmella L.var oleraceae Clarke, S. calva L. and S. paniculata Wall ex DC. Cent percent mortalities was achieved against the late third/early fourth instar larvae of A. stephensi Liston, A. culicifacies species C and C. quinquefasciatus Say using crude hexane extract obtained from flower heads of Spilanthes spp. Of the three plant species, S. acmella extract proved to be the most effective in inducing complete lethality at minimum doses, the respective LC50 and LC90 values being 4.57 and 7.83 (A. stephensi), 0.87 and 1.92 (A. culicifacies) and 3.11and 8.89 ppm (C. quinquefasciatus). This was followed by S. calva and S. paniculata extracts, respectively. This is the first report of achieving cent percent lethality against these mosquito larvae using minimal doses of plant extracts from this or any other plant species.

References

  1. Anonymous (1989) The wealth of India: a dictionary of Indian raw materials and industrial products, vol. 10. Publication and Information Directorate, CSIR, New Delhi, India, pp 11–12Google Scholar
  2. Borges-Del-Castillo J, Vazquez-Bueno P, Secundino-Lucas M, Martinez-Martir I, Joseph-Nathan P (1984) The N-2-phenylethylcinnamide from Spilanthes ocymifolia. Phytochemistry 23:2671–2672CrossRefGoogle Scholar
  3. Fabry W, Okemo PO, Ansong R (1996) Fungistatic and fungicidal activity of east African medicinal plants. Mycoses 39:67–70PubMedCrossRefGoogle Scholar
  4. Fabry W, Okemo PO, Ansong R (1998) Antibacterial activity of East African Medicinal plants. J Ethnopharmacology 60:79–84CrossRefGoogle Scholar
  5. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, UK, pp 1–333Google Scholar
  6. Jondiko IJO (1986) A mosquito larvicide in Spilanthes mauritiana. Phytochemistry 25:2289–2290CrossRefGoogle Scholar
  7. Kannathasan K, Senthilkumar A, Chandrasekaran M, Venkatesalu V (2007) Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae. Parasitol Res (in press)Google Scholar
  8. Nayak S, Chand R (2002) Dynamics of spilanthol in Spilanthes acmella Murr. in plant growth and development. Indian Drugs 39(8):419–421Google Scholar
  9. Pendse GS, Phalnikar NL, Bhide BV (1945) Investigation of new plant larvicides with special reference to Spilanthes acmella. Curr Sci 2:37–38Google Scholar
  10. Pitasawat B, Choochote W, Kanjanapothi D, Panthong A, Jitpakdi A, Chaithong U (1998) Screening for larvicidal activity of ten carminative plants. Southeast Asian J Trop Med Public Health 29:660–662PubMedGoogle Scholar
  11. Raghavendra K, Subbarao SK (2002a) Case studies on insecticide resistance and its management. In: Frederick G (ed) Proceedings of Mekong Malaria Forum, RMCP-EC, pp 17–21Google Scholar
  12. Raghavendra K, Subbarao SK (2002b) Chemical insecticide in malaria vector control in India. ICMR Bull 32:93–99Google Scholar
  13. Rahman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71:553–555CrossRefGoogle Scholar
  14. Rai M, Varma A, Pandey K (2004) Antifungal potentials of Spilanthes calva after inoculation of Piriformospora indica. Mycoses 47(112):479–481PubMedCrossRefGoogle Scholar
  15. Ramsewak RS, Erickson AJ, Nair MG (1999) Bioactive N-isobutylamides from the flower buds of Spilanthes acmella. Phytochemistry 51:729–732PubMedCrossRefGoogle Scholar
  16. Saraf DK, Dixit VK (2002) Spilanthes acmella Murr.: study on its extract spilanthol as larvicidal compound. Asian J Exp Sci 16:9–19Google Scholar
  17. Siddiqui BS, Afshan F, Gulzar T, Sultana R, Naqvi SN, Tariq RM (2003) Tetracyclic triterpenoids from the leaves of Azadirachta indica and their insecticidal activities. Chem Pharm Bull 51:415–417PubMedCrossRefGoogle Scholar
  18. Sukumar K, Perich MJ, Boobar LR (1991) Botanical derivatives in mosquito control: a review. J Am Mosq Control Assoc 7:210–237PubMedGoogle Scholar
  19. World Health Organization (1981) Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides, WHO/VBC/81.807. WHO, GenevaGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Vibha Pandey
    • 1
  • Veena Agrawal
    • 1
  • K. Raghavendra
    • 2
  • A. P. Dash
    • 2
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.National Institute of Malaria ResearchDelhiIndia

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