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Parasitology Research

, Volume 102, Issue 2, pp 277–282 | Cite as

Gene expression profile of Clonorchis sinensis metacercariae

  • Pyo Yun Cho
  • Tae Im Kim
  • Seong Man Whang
  • Sung-Jong HongEmail author
Original Paper

Abstract

Clonorchis sinensis develop through miracidium, sporocyst, redia, cercaria, and metacercaria stages before becoming egg-laying adult flukes. The authors undertook this analysis of gene expression profiles during developmental stages to increase our understanding of the biology of C. sinensis and of host–parasite relationships. From a C. sinensis metacercariae complementary deoxyribonucleic acid library, 419 expressed sequence tags (ESTs) of average length of 668 bp were collected and assembled into 322 genes containing 70 clusters and 252 singletons. The genes were annotated using BLAST searches and categorized into ten major functional categories. Genes expressed abundantly were those of proteases and metabolic, transcription, and translation housekeeping proteins. Genes expressed higher in C. sinensis metacercariae than in adults coded structural and cytoskeletal proteins, transcription and translation machinery proteins, and energy metabolism-related proteins. This EST information supports the notion that C. sinensis metacercariae in fish hosts have a physiology and metabolism that is quite different from that of its adult form in mammals.

Keywords

Bile Acid FMRFamide Fumarate Hydratase Complementary Deoxyribonucleic Acid Adult Fluke 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by the Korean Science and Engineering Foundation (Grant no. M2-0408-00-0046).

References

  1. Andrews P (1985) Praziquantel: mechanisms of anti-schistosomal acitivity. Pharmacol Ther 29:129–156PubMedCrossRefGoogle Scholar
  2. Chen YH, Li MH, Zhang Y, He LL, Yamada Y, Fitzmaurice A, Shen Y, Zhang H, Tong L, Yang J (2004) Structural basis of the a1-b subunit interaction of voltage-gated Ca2+ channels. Nature 429:675–680PubMedCrossRefGoogle Scholar
  3. Cho PY, Lee MJ, Kim TI, Kang SY, Hong SJ (2006) Expressed sequence tag analysis of adult Clonorchis sinensis, the Chinese liver fluke. Parasitol Res 99:602–608PubMedCrossRefGoogle Scholar
  4. Davey F, Harris SJ, Cottrell GA (2001) Histochemical localization of FMRFamide-gated Na+ channels in Helisoma trivolvis and Helix aspersa neurons. J Neurocytel 30:877–884CrossRefGoogle Scholar
  5. Doyle DA, Cabral JM, Pfuetzner RA, Kuo A, Gulbis JM, Dohen SL, Chair BT, MacKinnon R (1998) The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science 280:69–77PubMedCrossRefGoogle Scholar
  6. Green KA, Cottrell GA (1999) Block of the helix FMRFamide-gated Na+ channel by FMRFamide and its analogues. J Physiol 519:47–56PubMedCrossRefGoogle Scholar
  7. Haas BJ, Berriman M, Hirai H, Cerqueira GG, LoVerde PT, El-Sayed NM (2007) Schistosoma mansoni genome: closing in on a final gene set. Exp Parasitol (in press), DOI  10.1016/j.expara.2007.06.005
  8. Hong SJ, Seong KY, Sohn WM, Song KY (2000) Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis. Mol Biochem Parasitol 108:207–216PubMedCrossRefGoogle Scholar
  9. Hu W, Yan Q, Shen DK, Liu F, Zhu ZD, Song HD, Xu XR, Wang ZJ, Rong YP, Zeng LC, Wu J, Zhang X, Wang JJ, Xu XN, Wang SY, Fu G, Zhang XL, Wang ZQ, Brindley PJ, McManus DP, Xue CL, Feng Z, Chen Z, Han ZG (2003) Evolutionary and biomedical implications of a Schistosoma japonicum complementary DNA resource. Nat Genet 35:139–147PubMedCrossRefGoogle Scholar
  10. Jeziorski MC, Greenberg RM (2006) Voltage-gated calcium channel subunits from platyhelminths: potential role in praziquantel action. Int J Parasitol 36:625–632PubMedCrossRefGoogle Scholar
  11. Jeziorski MC, Green KA, Sommerville J, Cottrell GA (2000) Cloning and expression of a FMRFamide-gated Na+ channel from Helisoma trivolvis and comparison with the native neuronal channel. J Physiol 526:13–25PubMedCrossRefGoogle Scholar
  12. Kim TS, de Guzman JV, Kong HH, Chung DI (2006) Comparison of gene representation between diploid and triploid Paragonimus westermani by expressed sequence tag analyses. J Parasitol 92:803–816PubMedCrossRefGoogle Scholar
  13. Kohn AB, Anderson PAV, Roberts-Misterly JM, Greenberg RM (2001) Schistosome calcium channel b . subunits. : unusual modulatory effects and potential role in the action of the antischistosomal drug praziquantel. J Biol Chem 276:36873–36876PubMedCrossRefGoogle Scholar
  14. Laha T, Pinlaor P, Mulvenna J, Sripa B, Sripa M, Smout MJ, Gasser RB, Brindley PJ, Loukas A (2007) Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini. BMC Genomics 8:189PubMedCrossRefGoogle Scholar
  15. Lee JS, Lee JW, Park SJ, Yong TS (2003) Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach. Parasitol Res 91:283–289PubMedCrossRefGoogle Scholar
  16. Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, Fang Y (2005) Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect Dis 5:31–41PubMedCrossRefGoogle Scholar
  17. Marks NJ, Halton DW, Maule AG, Brennan GP, Shaw C, Southgate VR, Johnston CF (1995) Comparative analyses of the neuropeptide F (NPF)- and FMRFamide-related peptide (FaRP)-immunoreactivities in Fasciola hepatica and Schistosoma spp. Parasitology 11(pt4):371–381CrossRefGoogle Scholar
  18. Matern H, Heinemann H, Legler G, Matern S (1997) Purification and characterization of a microsomal bile acid beta-glucosidase from human liver. J Biol Chem 272:11261–11267PubMedCrossRefGoogle Scholar
  19. Matern H, Boermans H, Lottspeich F, Matern S (2001) Molecular cloning and expression of human bile acid beta-glucosidase. J Biol Chem 276:37929–37933PubMedGoogle Scholar
  20. Merrick JM, Osman A, Tsai J, Loverde PT, Lee NH (2003) The Schistosoma mansoni gene index: Gene discovery and biology by reconstruction and analysis of expressed gene sequences. J Parasitol 89:261–269PubMedCrossRefGoogle Scholar
  21. Momose T, Maruyama J, Iida T, Goto J, Nambara T (1997) Comparative abilities and optimal conditions for b-. glucosidase enzymes to hydrolyse the glucuronide, glucoside, and . N-acetylglucosaminide conjugates of bile acids. Biol Pharm Bull 20:828–833PubMedGoogle Scholar
  22. Nelson LS, Rosoff ML, Li C (1998) Disruption of a neuropeptide gene, flp-1, causes multiple behavioral defects in Caenorhabditis elegans. Science 281:1686–1690PubMedCrossRefGoogle Scholar
  23. Perry SJ, Straub VA, Schofield MG, Schofield MG, Burke JF, Benjamin PR (2001) Neutonal expression of an FMRFamide-gated Na+ channel and its modulation by acid pH. J Neurosci 21:5559–5567PubMedGoogle Scholar
  24. Rim HJ (2005) Clonorchiasis: an update. J Helminthol 79:269–281PubMedCrossRefGoogle Scholar
  25. Rim HJ, Ha JH, Kim SJ (1980) Experimental study on the therapeutic effect of praziquantel (Embay 8440) in rats infected with Clonorchis sinensis. Korean J Parasitol 18:65–80CrossRefGoogle Scholar
  26. Squatrio M, Mancino M, Donzelli M, Areces LB, Draetta GF (2004) EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes. Oncogene 23:4454–4465CrossRefGoogle Scholar
  27. Verjovski-Almeida S, DeMarco R, Martins EAL, Guimaraes PEM, Ojopi EPB, Paquola ACM, Piazza JP, Nishiyama MY Jr, Kitajima J, Adamson RE, Ashton PD, Bonaldo MF, Coulson PS, Dillon GP, Farias LP, Gregorio SP, Ho PL, Leite RA, Malaquias LCC, Marques RCP, Miyasato PA, Nascimento ALTO, Ohlweiler FP, Reis EM, Ribeiro MA, Sa RG, Stukart GC, Soares MB, Gargioni C, Kawano T, Rodrigues V, Madeira AMBN, Wilson RA, Menck CFM, Setubal JC, Leite LCC, Dias-Neto E (2003) Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni. Nat Genet 35:148–157PubMedCrossRefGoogle Scholar
  28. Zhang Y, Hamberger AW (2004) Heregulin regulates the ability of the ErbB3-binding protein Ebp1 to bind E2F promoter elements and repress E2F-mediated transcription. J Biol Chem 279:26126–26133PubMedCrossRefGoogle Scholar
  29. Zhang Y, Woodford N, Xia X, Hamberger AW (2003) Repression of E2F1-mediated transcription by the ErbB3 binding protein Ebp1 involves histone deacetylases. Nucleic Acids Res 31:2168–2177PubMedCrossRefGoogle Scholar
  30. Zhang Y, Akinmade D, Hamberger AW (2005) The ErbB3 binding protein Ebp1 interacts with Sin3A to repress E2F1 and AR-mediated transcription. Nucleic Acids Res 33:6024–6033PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Pyo Yun Cho
    • 1
    • 2
  • Tae Im Kim
    • 1
  • Seong Man Whang
    • 1
  • Sung-Jong Hong
    • 1
    Email author
  1. 1.Department of Parasitology, College of MedicineChung-Ang UniversitySeoulSouth Korea
  2. 2.Division of Malaria and Parasitic Diseases, Centers for Disease Control and PreventionNational Institute of HealthSeoulSouth Korea

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