Skip to main content
Log in

Passalurus ambiguus: new insights into copromicroscopic diagnosis and circadian rhythm of egg excretion

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The present paper reports a study on the in vivo diagnosis and egg excretion rhythm of the pinworm Passalurus ambiguus in domesticated rabbits. Three copromicroscopic techniques were compared: the cellophane tape test, the McMaster technique, and the FLOTAC technique. Out of the 51 New Zealand White rabbit does examined, 42 (82.3%) resulted positive when examined with the FLOTAC technique, 39 (76.5%) with the cellophane tape test, and 29 (56.9%) with the McMaster technique. The agreement between the FLOTAC technique and the cellophane tape test was almost perfect (greater than 0.8); only moderate were the agreements (0.4) between the FLOTAC and the McMaster techniques and between the McMaster technique and the cellophane tape test. The results showed that the FLOTAC technique can be used for the quali–quantitative coprological diagnosis of P. ambiguus in rabbits due to its great sensitivity, as already shown for parasites of other animal species. The circadian rhythm of egg excretion by P. ambiguus was studied utilizing 42 individually caged rabbit does; fecal samples were collected from each cage every 6 h, i.e., at 6:00–12:00 hours, 12:00–18:00 hours, 18:00–24:00 hours, and 24:00–6:00 hours, and were analyzed by the FLOTAC technique. A circadian rhythm of P. ambiguus egg excretion was found, with significant lower values at 6:00–12:00 hours. In conclusion, the present study showed that the FLOTAC technique is the best copromicroscopic method for assessing P. ambiguus prevalence and intensity in rabbits and that the afternoon and evening hours are the best times for fecal sampling to perform the pinworm diagnosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Allan JC, Craig PS, Sherington J, Rogan MT, Storey DM, Heath S, Iball K (1999) Helminth parasites of the wild rabbit Oryctolagus cuniculus near Malham Tarn, Yorkshire, UK. J Helminthol 73:289–294

    PubMed  CAS  Google Scholar 

  • Boag B (1985) The incidence of helminth parasites from the wild rabbit Oryctolagus cuniculus (L) in eastern Scotland. J Helminthol 59:61–69

    Article  PubMed  CAS  Google Scholar 

  • Boag B (1988) Observations on the seasonal incidence of myxomatosis and its interactions with helminth parasites in the European rabbit (Oryctolagus cuniculus). J Wildl Dis 24:450–455

    PubMed  CAS  Google Scholar 

  • Boag B, Lello J, Fenton A, Tompkins DM, Hudson PJ (2001) Patterns of parasite aggregation in the wild European rabbit (Oryctolagus cuniculus). Int J Parasitol 31:1421–1428

    Article  PubMed  CAS  Google Scholar 

  • Colin M, Lebas F (1996) Rabbit meat production in the world. A proposal for every country. In: Proc. 6th World Rabbit Cong., Toulouse, France, pp 323–330

  • Cringoli G (2006) FLOTAC, a novel apparatus for a multivalent faecal egg count technique. Parassitologia 48:385–389

    Google Scholar 

  • Cringoli G, Rinaldi L, Veneziano V, Capelli G, Scala A (2004) The influence of flotation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Vet Parasitol 123:121–131

    Article  PubMed  CAS  Google Scholar 

  • Epe C, Coati N, Schnieder T (2004) Results of parasitological examinations of faecal samples from horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002. Dtsch Tierarztl Wochenschr 111:243–247

    PubMed  CAS  Google Scholar 

  • Falconer DS (1989) Introduction to quantitative genetics, 3rd edn. Longman Scientific and Technical Press, London, UK

    Google Scholar 

  • Felt SA, White CE (2005) Evaluation of a timed and repeated perianal tape test for the detection of pinworms (Trypanoxyuris microon) in owl monkeys (Aotus nancymae). J Med Primatol 34:209–214

    Article  PubMed  CAS  Google Scholar 

  • Foronda P, Del Castillo A, Abreu N, Figueruelo E, Pinero J, Casanova JC (2003a) Parasitic helminths of the wild rabbit, Oryctolagus cuniculus, in different bioclimatic zones in Tenerife, Canary Islands. J Helminthol 77:305–309

    Article  PubMed  CAS  Google Scholar 

  • Foronda P, Valladares B, Lorenzo-Morales J, Ribas A, Feliu C, Casanova JC (2003b) Helminths of the wild rabbit (Oryctolagus cuniculus) in Macaronesia. J Parasitol 89:952–957

    Article  PubMed  CAS  Google Scholar 

  • Grice RL, Prociv P (1993) In vitro embryonation of Syphacia obvelata eggs. Int J Parasitol 23:257–260

    Article  PubMed  CAS  Google Scholar 

  • Hobbs RP, Twigg LE, Elliot AD, Wheeler AG (1999a) Evaluation of the association of parasitism with mortality of wild European rabbits Oryctolagus cuniculus (L.) in southwestern Australia. J Parasitol 85:803–808

    Article  PubMed  CAS  Google Scholar 

  • Hobbs RP, Twigg LE, Elliot AD, Wheeler AG (1999b) Factors influencing the fecal egg and oocyst counts of parasites of wild European rabbits Oryctolagus cuniculus (L.) in Southern Western Australia. J Parasitol 85:796–802

    Article  PubMed  CAS  Google Scholar 

  • Jilge B, Stahle H (1984) The internal synchronization of five circadian functions of the rabbit. Chronobiol Int 1:195–204

    Article  PubMed  CAS  Google Scholar 

  • Kucik CJ, Martin GL, Sortor BV (2004) Common intestinal parasites. Am Fam Physician 69:1161–1168

    PubMed  Google Scholar 

  • MAFF (1986) Manual of veterinary parasitological laboratory techniques, reference book 418. Ministry of Agriculture, Fisheries and Food, HMSO, London, p 5

  • Mes TH (2003) Technical variability and required sample size of helminth egg isolation procedures. Vet Parasitol 115:311–320

    Article  PubMed  Google Scholar 

  • Ostler DC (1961) The diseases of broiler rabbits. Vet Rec 73:1237–1252

    PubMed  CAS  Google Scholar 

  • Owen D (1972) Common parasites of laboratory rodents and lagomorphs. Laboratory animals centre handbook no. 1. Medical research council

  • Quesada A, Cringoli G, Coppola C (1987) Primi risultati di un’indagine conoscitiva sulla presenza e tipizzazione dei nematelminti parassiti negli allevamenti cunicoli campani. Rivista di Coniglicoltura 1:37–40

    Google Scholar 

  • Parija SC, Sheeladevi C, Shivaprakash MR, Biswal N (2001) Evaluation of lactophenol cotton blue stain for detection of eggs of Enterobius vermicularis in perianal surface samples. Trop Doct 31:214–215

    PubMed  CAS  Google Scholar 

  • Pisanu B, Chapuis JL, Durette-Desset MC (2001) Helminths from introduced small mammals on Kerguelen, Crozet, and Amsterdam Islands (southern Indian Ocean). J Parasitol 87:1205–1208

    PubMed  CAS  Google Scholar 

  • Procop GW (2001) Gastrointestinal infections. Infect Dis Clin North Am 15:1073–1108

    Article  PubMed  CAS  Google Scholar 

  • Sapozhnikov GI (1969) The life cycle of Skrjabinema ovis (Skrjabin 1915). Trudi Vsesoyuznogo Instituta Gel’mintologii 15:267–274

    Google Scholar 

  • Shiibashi T, Imai T, Sato Y, Abe N, Yukawa M, Nogami S (2006) Cryptosporidium infection in juvenile pet rabbits. J Vet Med Sci 68:281–282

    Article  PubMed  Google Scholar 

  • Taffs LF (1976) Pinworm infections in laboratory rodents: a review. Lab Anim 10:1–13

    Article  PubMed  CAS  Google Scholar 

  • Thrusfield M (1995) Veterinary epidemiology. Blackwell, London, UK

    Google Scholar 

  • Villanua D, Perez-Rodriguez L, Gortazar C, Hofle U, Vinuela J (2006) Avoiding bias in parasite excretion estimates: the effect of sampling time and type of faeces. Parasitology 133:251–259

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Giuseppe Cringoli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rinaldi, L., Russo, T., Schioppi, M. et al. Passalurus ambiguus: new insights into copromicroscopic diagnosis and circadian rhythm of egg excretion. Parasitol Res 101, 557–561 (2007). https://doi.org/10.1007/s00436-007-0513-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-007-0513-z

Keywords

Navigation