Immunohistochemical study of the local immune response in lambs experimentally infected with Dicrocoelium dendriticum (Digenea)
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Phenotypic expression of inflammatory cells in liver and hepatic lymph nodes (HLN) has been examined in lambs experimentally infected with Dicrocoelium dendriticum using immunohistochemical techniques. Thirty-two lambs, 12 infected with 1,000 D. dendriticum metacercariae, 12 with 3,000, and 8 controls were used. Half the lambs in each group were slaughtered on days 60 and 180 post-infection (p.i.), respectively. Primary antibodies (Abs) against T cell epitopes (CD3+, CD4+, CD8+ and WC1+ γδ), B cell epitopes (CD79αcy+, CD45R+), immunoglobulin (IgG)-bearing plasma cells, macrophages (CD14+, VPM32+) and major histocompatibility complex (MHC) class IIβ antigen were used. T lymphocytes (CD3+, CD4+ and CD8+) and B lymphocytes (CD79αcy+ and CD45R+) with diffuse pattern or forming lymphoid aggregates and follicles surrounded the septal bile ducts (SBD) and inter-lobular bile ducts, whereas the WC1 γδ T cells were scattered. Numerous IgG+ plasma cells were observed around SBD. CD14 and VPM32+ macrophages intermingled with lymphocytes were immunostained by the anti-MHC class IIβ. This Ab also reacted with lymphoid cells. Likewise, increased positive immunostaining for all Abs used was observed in the HLN of infected lambs. There was no qualitative difference regarding the phenotype expression of inflammatory cells between the lambs infected with D. dendriticum. The humoral and cell-mediated local immune responses observed were similar in the two groups of lambs infected with different doses.
KeywordsBile Duct Major Histocompatibility Complex Class Cell Epitope Lymphoid Follicle Small Round Cell
We would like to thank M. P. del Pozo, C. Espiniella and M. L. Carcedo of the Estación Agrícola Experimental (CSIC, León, Spain) and S. Liébana and J. Benavides of the Departamento de Sanidad Animal de la Universidad de León (Spain) for their technical assistance. We also wish to thank P. Díez, L. Pérez and H. Fidalgo for taking care of and handling the animals (CSIC, León). This study was supported by the Spanish CICYT (Projects No. AGF92-0588 and AGF96-0416) and by the “Junta de Castilla y León” (Project No. CSIC01A06). The experiments carried out with lambs in this study comply with current Spanish law on animal experimentation.
- Aould J (1994) Antigen unmasking in routinely processed paraffin sections by pressure cooking. United Kingdom National External Quality Assessment Schemes—ICC, pp 6–9Google Scholar
- Gershwin LJ, Krakowka S, Olsen RG (1995) Immunology and immunopathology of domestic animals. Mosby, St. Louis, MO, pp 62–67Google Scholar
- Isse K, Harada K, Sato Y, Nakanuma Y (2006) Characterization of biliary intra-epithelial lymphocytes at different anatomical levels of intrahepatic bile ducts under normal and pathological conditions: Numbers of CD4+CD28-intra-epithelial lymphocytes are increased in primary biliary cirrhosis. Pathol Int 56:17–24PubMedCrossRefGoogle Scholar
- Pérez J, Martín de las Mulas J, Chacón-M De Lara F, Gutiérrez-Palomino PN, Becerra-Martel C, Martínez-Moreno A (1998) Immunohistochemical study of the local immune response to Fasciola hepatica in primarily and secondarily infected goats. Vet Immunol Immunopathol 64:337–348PubMedCrossRefGoogle Scholar
- Pérez J, Ortega J, Moreno T, Morrondo P, López-Sánchez C, Martínez-Moreno A (2002) Pathological and immunohistochemical study of the liver and hepatic lymph nodes of sheep chronically reinfected with Fasciola hepatica, with or without triclabendazole treatment. J Comp Path 127:30–36PubMedCrossRefGoogle Scholar
- Rimmelzwann GF, Osterhaus ADME (1999) The immune response. In: Pastoret PP, Blancou J, Vannier P, Verschueren C (eds) Veterinary vaccinology. Elsevier, Amsterdam, pp 55–64Google Scholar
- Timoteo O, Maco V Jr, Maco V, Neyra V, Yi PJ, Leguía G, Espinoza JR (2005) Characterization of the humoral immune response in alpacas (Lama pacos) experimentally infected with Fasciola hepatica against cysteine proteinases Fas1 and Fas2 and histopathological findings. Vet Immunol Immunopathol 106:77–86PubMedGoogle Scholar
- Vray B (2002) Macrophages in parasitic infection. In: Burke B, Lewis CE (eds) The macrophage. Oxford Univ. Press, Oxford, UK pp 253–304Google Scholar