Parasitology Research

, Volume 101, Issue 3, pp 547–555 | Cite as

Immunohistochemical study of the local immune response in lambs experimentally infected with Dicrocoelium dendriticum (Digenea)

  • M. Carmen Ferreras-EstradaEmail author
  • R. Campo
  • C. González-Lanza
  • V. Pérez
  • J. F. García-Marín
  • M. Y. Manga-González
Original Paper


Phenotypic expression of inflammatory cells in liver and hepatic lymph nodes (HLN) has been examined in lambs experimentally infected with Dicrocoelium dendriticum using immunohistochemical techniques. Thirty-two lambs, 12 infected with 1,000 D. dendriticum metacercariae, 12 with 3,000, and 8 controls were used. Half the lambs in each group were slaughtered on days 60 and 180 post-infection (p.i.), respectively. Primary antibodies (Abs) against T cell epitopes (CD3+, CD4+, CD8+ and WC1+ γδ), B cell epitopes (CD79αcy+, CD45R+), immunoglobulin (IgG)-bearing plasma cells, macrophages (CD14+, VPM32+) and major histocompatibility complex (MHC) class IIβ antigen were used. T lymphocytes (CD3+, CD4+ and CD8+) and B lymphocytes (CD79αcy+ and CD45R+) with diffuse pattern or forming lymphoid aggregates and follicles surrounded the septal bile ducts (SBD) and inter-lobular bile ducts, whereas the WC1 γδ T cells were scattered. Numerous IgG+ plasma cells were observed around SBD. CD14 and VPM32+ macrophages intermingled with lymphocytes were immunostained by the anti-MHC class IIβ. This Ab also reacted with lymphoid cells. Likewise, increased positive immunostaining for all Abs used was observed in the HLN of infected lambs. There was no qualitative difference regarding the phenotype expression of inflammatory cells between the lambs infected with D. dendriticum. The humoral and cell-mediated local immune responses observed were similar in the two groups of lambs infected with different doses.


Bile Duct Major Histocompatibility Complex Class Cell Epitope Lymphoid Follicle Small Round Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to thank M. P. del Pozo, C. Espiniella and M. L. Carcedo of the Estación Agrícola Experimental (CSIC, León, Spain) and S. Liébana and J. Benavides of the Departamento de Sanidad Animal de la Universidad de León (Spain) for their technical assistance. We also wish to thank P. Díez, L. Pérez and H. Fidalgo for taking care of and handling the animals (CSIC, León). This study was supported by the Spanish CICYT (Projects No. AGF92-0588 and AGF96-0416) and by the “Junta de Castilla y León” (Project No. CSIC01A06). The experiments carried out with lambs in this study comply with current Spanish law on animal experimentation.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • M. Carmen Ferreras-Estrada
    • 1
    Email author
  • R. Campo
    • 2
  • C. González-Lanza
    • 2
  • V. Pérez
    • 1
  • J. F. García-Marín
    • 1
  • M. Y. Manga-González
    • 2
  1. 1.Departamento de Sanidad Animal (Histología y Anatomía Patológica), Facultad de VeterinariaUniversidad de LeónLeonSpain
  2. 2.Consejo Superior de Investigaciones Científicas (CSIC)Estación Agrícola ExperimentalLeonSpain

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