Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis
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With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bacteria (HP6-GST [glutathione S-transferase]), employing enzyme-linked immunosorbent assays (ELISAs) and sera from T. saginata infected cattle, T. solium infected pigs and serum and cerebrospinal fluid (CSF) samples from clinically defined T. solium neurocysticercosis (NCC) patients. The two recombinant proteins were antigenic in all three systems, with the signal to background ratio of the HP6-Bac ELISA slightly higher than that for the HP6-GST ELISA. Assay performance in cattle was similar to previously described peptide-based ELISA assays, although NCC sample sensitivity/specificity was marginally better. The sensitivity of the HP6-Bac and HP6-GST ELISAs was close for active human NCC (77.4 and 80.6% for serum and 76.9 and 73.1% for CSF samples, respectively). In inactive human NCC, however, the sensitivity of the HP6-Bac ELISA was almost twice that of the HP6-GST ELISA. Because peptides are relatively expensive and recombinant proteins are simple and economical to produce, the latter may provide useful reagents for antibody detection in countries with endemic cysticercosis/NCC.
KeywordsRecombinant Protein Cysticercosis Baculovirus System Taenia Solium Porcine Cysticercosis
This investigation received financial support from EU/INCO-DC (project CT95-0002), FIS (00/40702/1114) and ISCIII-Programa Intramural. Elizabeth Ferrer was supported by grants from AECI, ISCIII and RICET-FIS (RD 06/0021/0019). The authors would like to thank Maria Lares, Glenda Rojas, Yenny Alviarez (BIOMED) and Mercedes Rodríguez (ISCIII) for their collaboration in the collection of human serum samples. We also would like to thank Paloma Resino (INIA) for her collaboration in larvae handling. The experiments described in this paper comply with the current laws of Venezuela and Spain, the countries in which the work was performed.
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