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Parasitology Research

, Volume 101, Issue 2, pp 259–268 | Cite as

Ultrastructure of spermiogenesis and the spermatozoon of Paramphistomum microbothrium (Fischoeder 1901; Digenea, Paramphistomidae), a parasite of Bos taurus in Senegal

  • Momar Talla SeckEmail author
  • Bernard Marchand
  • Cheikh Tidiane Bâ
Original Paper

Abstract

The ultrastructural study of spermiogenesis and the spermatozoon of Paramphistomum microbothrium gave evidence of some characteristics of this digenean. The intercentriolar body located between two striated roots and two centrioles exhibits a symmetric plane. The two external bands of this intercentriolar body are made up of a line of granules. The striated roots are about 3.7-μm long. During spermiogenesis, a flagellar rotation of 90° is described. The spermatozoon is characterized in its anterior part by the existence of external ornamentations and a lateral expansion exhibiting a “spine-like body.” Such a spine-like body has never been described before in a digenean. The region II of the spermatozoon is asymmetrical and also characterized by the absence of mitochondrion. The posterior end of this spermatozoon exhibits a nucleus and a few microtubules.

Keywords

Lateral Expansion Mature Spermatozoon Cortical Microtubule Differentiation Zone Anterior Extremity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Baptista-Farias MFD, Kohn A, Cohen SC (2001) Ultrastructure of spermatogenesis and sperm development in Saccocoeliodes godoyi Kohn & Froes, 1896 (Digenea, Haploporidae). Mem Inst Oswaldo Cruz 96:61–70PubMedCrossRefGoogle Scholar
  2. Burton PR (1972) Fine structure of the reproductive system of a frog lung fluke, III. The spermatozoon ands its differentiation. J Parasitol 58:68–83PubMedCrossRefGoogle Scholar
  3. Castilho F, Barandela T (1990) Ultrastructural study on the spermiogenesis and spermatozoon of the metacercariae of Microphallus primas (Digenea), a parasite of Carcinus maenas. Mol Reprod Dev 25:140–146PubMedCrossRefGoogle Scholar
  4. Cifrian B, Garcia-Corrales P, Martinez Alos S (1993) Ultrastructural study of the spermatogenesis and mature spermatozoa of Dicrocoelium dendriticum (Platyhelminthes, Digenea). Parasitol Res 79:204–212PubMedCrossRefGoogle Scholar
  5. Ehlers U (1984) Phylogenetisches system der Platyhelminthes. Verh Naturwiss Ver Hambg 27:291–294Google Scholar
  6. Erwin BE, Halton DW (1983) Fine structural observations on spermatogenesis in a progenetic Trematode, Bucephaloides gracilescens. Int J Parasitol 13:413–426CrossRefGoogle Scholar
  7. Fujino T, Ishii Y (1982) Ultrastructural studies on spermatogenesis in a parthenogenetic type of Paragonimus westermani (Kebert 1878) proposed as P. pulmonalis (Baelz 1880). J Parasitol 68:433–441PubMedCrossRefGoogle Scholar
  8. Fujino T, Ishii Y, Mori T (1977) Ultrastructural studies on the spermatozoa and spermatogenesis in Paragonimus and Eurytrema (Trematoda: Digenea). Jap J Parasitol 26:240–255Google Scholar
  9. Gracenea M, Ferrer JR, Gonzalez-Moreno O, Trullols M (1997) Ultrastructural study of spermatogenesis and the spermatozoon in Postorchigenes gymnesicus (Trematoda, Lecithodendriidae). J Morphol 234:223–232CrossRefGoogle Scholar
  10. Gracenea M, Ferrer J, Gonzalez-Moreno O (2000) The spermatogenesis and the spermatozoon in Brachylaima sp. (Digenea): ultrastructural study. Acta Parasitol 45:169Google Scholar
  11. Grant WC, Harkema R, Muse KE (1976) Ultrastructure of Pharyngostomoides procyonis Harkema 1942 (Diplostomatidae), I. Observations on the male reproductive system. J Parasitol 62:39–49PubMedCrossRefGoogle Scholar
  12. Hendow HT, James BL (1988) Ultrastructure of spermatozoon and spermatogenesis in Maritrema linguilla (Digenea: Microphallidae). Int J Parasitol 18:53–63PubMedCrossRefGoogle Scholar
  13. Hirai H, Tada I (1991) Morphological features of spermatozoa of Paragonimus ohirai (Trematoda: Platyhelminthes) examined by a silver nitrate staining technique. Parasitology 103:103–110PubMedGoogle Scholar
  14. Iomini C, Justine J-L (1997) Spermiogenesis and spermatozoon of Echinostoma caproni (Platyhelminthes, Digenea): transmission and scanning electron microscopy, and tubulin immunocytochemistry. Tissue Cell 29:107–118PubMedCrossRefGoogle Scholar
  15. Iomini C, Mollaret I, Albaret JL, Justine J-L (1997) Spermatozoon and spermiogenesis in Mesocoelium monas (Platyhelminthes: Digenea): ultrastructure and epifluorescence microscopy of labelling of tubulin and nucleus. Folia Parasitol 44:26–32PubMedGoogle Scholar
  16. Jamieson BGM, Daddow LYM (1982) The ultrastructure of the spermatozoon of Neochasmus sp. (Cryptogonimidae, Digenea, Trematoda) and its phylogenetic significance. Int J Parasitol 12:547–559CrossRefGoogle Scholar
  17. Jamieson BGM, Daddow LYM (1983) An ultrastructural study of spermiogenesis in Neochasmus sp. (Cryptogonimidae: Digenea :Trematoda). Aust J Zool 31:1–14CrossRefGoogle Scholar
  18. Justine J-L (1995) Spermatozoal ultrastructure and phylogeny in the parasitic Platyhelminthes. Mem Mus Natl Hist Nat Paris 166:55–86Google Scholar
  19. Justine J-L, Mattei X (1981) Etude ultrastructurale du flagelle spermatiques des Schistosomes (Trematoda : Digenea). J Ultrastruct Res 76:89–95PubMedCrossRefGoogle Scholar
  20. Justine J-L, Mattei X (1982a) Etude ultrastructurale de la spermiogenèse et du spermatozoïde d’un Platyhelminthe: Gonapodasmius (Trematoda: Didymozoidae). J Ultrastruct Res 79:350–365PubMedCrossRefGoogle Scholar
  21. Justine J-L, Mattei X (1982b) Réinvestigation de l’ultrastructure du spermatozoïde d’Haematoloechus (Trematoda: Haematoloechidae). J Ultrastruct Res 81:322–332PubMedCrossRefGoogle Scholar
  22. Justine J-L, Mattei X (1983) A spermatozoon with two 9 + 0 axonemes in a parasitic flatworm, Didymozoon (Digenea: Didymozoidae). J Submicrosc Cytol 15:1101–1105Google Scholar
  23. Justine J-L, Mattei X (1984a) Atypical spermiogenesis in a parasitic flatworm, Didymozoon (Trematoda: Digenea: Didymozoidae). J Ultrastruct Res 87:106–111CrossRefGoogle Scholar
  24. Justine J-L, Mattei X (1984b) Ultrastructural observations on the spermatozoon, ovocyte and fertilization process in Gonapodasmius, a gonochoristic trematode (Trematoda: Digenea: Didymozoidae). Acta Zool (Stockh) 65:215–221CrossRefGoogle Scholar
  25. Justine J-L, Jamieson BGM, Southgate VR (1993) Homogeneity of sperm structure in six species of Schistosomes (Digenea, Platyhelminthes). Ann Parasitol Hum Comp 68:185–187Google Scholar
  26. Kitajima EW, Paraense WL, Correa LR (1976) The fine structure of Schistosoma mansoni sperm (Trematoda: Digenea). J Parasitol 62:215–221PubMedCrossRefGoogle Scholar
  27. Levron C, Ternengo S, Marchand B (2003) Ultrastructure of spermiogenesis and the spermatozoon of Helicometra fasciata (Digenea, Opecoelidae), a parasite of Labrus merula (Pisces, Teleostei). Acta Parasitol 48:255–264Google Scholar
  28. Levron C, Ternengo S, Marchand B (2004a) Ultrastructure of spermiogenesis and the spermatozoon of Monorchis parvus Loss, 1902 (Digenea, Opecoelidae), a parasite of Diplodus annularis (Pisces, Teleostei). Parasitol Res 93:102–110PubMedCrossRefGoogle Scholar
  29. Levron C, Ternengo S, Marchand B (2004b) Spermiogenesis and sperm ultrastructure of Proracanthium furcatum (Digenea, Opecoelidae), a parasite of Mullus surmuletus (Pisces, Teleostei). Acta Parasitol 49:190–200Google Scholar
  30. Li M-M, Wang X-Y (1997) Spermatogenesis and ultrastructure of the metaphase chromosomes in Ceylonocotyle scoliocoelium (Digenea : Paramphistomidae). Acta Zool Sin 43:1–9Google Scholar
  31. Miquel J, Nourrisson C, Marchand B (2000) Ultrastructure of spermiogenesis and the spermatozoon of Opecoeloides furcatus (Trematoda, Digenea, Opecoelidae), a parasite of Mullus barbatus (Pisces, Teleostei). Parasitol Res 86:301–310PubMedCrossRefGoogle Scholar
  32. Morseth DJ (1969) Spermtail fine structure of Echinococcus granulosus and Dicrocoelium dendriticum. Exp Parasitol 24:47–53PubMedCrossRefGoogle Scholar
  33. Ndiaye PI, Miquel J, Bâ CT, Feliu C, Marchand B (2002) Spermiogenesis and sperm ultrastructure of Scaphiostomum palaearcticum Mas-Coma, Esteban et Valero, 1986 (Trematoda, Digenea, Brachylaimidae). Acta Parasitol 47:259–271Google Scholar
  34. Ndiaye PI, Miquel J, Feliu C, Marchand B (2003a) Ultrastructure of spermiogenesis and spermatozoa of Notocotylus neyrai Gonzalez castro, 1945 (Digenea, Notocotylidae), intestinal parasite of Microtus agrestis (Rodentia: Arvicolidae) in Spain. Invertebr Reprod Dev 43:105–115Google Scholar
  35. Ndiaye PI, Miquel J, Fons R, Marchand B (2003b) Spermiogenesis and sperm ultrastructure of the liver fluke Fasciola hepatica Linnaeus, 1758 (Digenea, Fasciolidae) : transmission and scanning electron microscopy, and tubulin immunocytochemistry. Acta Parasitol 48:182–194Google Scholar
  36. Ndiaye PI, Miquel J, Bâ CT, Marchand B (2004) Spermiogenesis and ultrastructure of the spermatozoon of the liver fluke Fasciola gigantica Cobbold, 1856 (Digenea: Fasciolidae), a parasite of cattle in Senegal. J Parasitol 90:30–40PubMedCrossRefGoogle Scholar
  37. Orido Y (1988) Ultrastructure of spermatozoa of the lung fluke, Paragonimus ohirai (Trematoda: Troglotrematidae), in seminal receptacle. J Morphol 196:333–334PubMedCrossRefGoogle Scholar
  38. Pamplona-Basilio MC, Baptista-Farias MFD, Kohn A (2001) Spermatogenesis and spermiogenesis in Didymocystis wedli Ariola, 1902 (Didymozoidae, Digenea). Mem Inst Oswaldo Cruz 96:1153–1159PubMedGoogle Scholar
  39. Rees FG (1979) The ultrastructure of the spermatozoon and spermiogenesis in Cryptocotyle lingua (Digenea: Heterophyidae). Int J Parasitol 9:405–419CrossRefGoogle Scholar
  40. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212PubMedCrossRefGoogle Scholar
  41. Robinson RD, Halton DW (1982) Fine structural observations on spermatogenesis in Corrigia vitta (Trematoda: Dicrocoeliidae). Z Parasitenkd 68:53–72CrossRefGoogle Scholar
  42. Sato M, Oh M, Sakoda K (1967) Electron microscopic study of spermatogenesis in the lung fluke (Paragonimus miyazakii). Z Zelforsch 77:232–243CrossRefGoogle Scholar
  43. Sharma PN, Rai N (1995) Ultrastructural study on spermatogenesis in Ganeo tigrinum, an intestinal trematode of Rana tigrina. J Helminthol 69:77–84CrossRefGoogle Scholar
  44. Stitt AW, Fairweather I (1990) Spermatogenesis and the fine structure of the mature spermatozoon of the liver fluke, Fasciola hepatica (Trematoda: Digenea). Parasitology 101:395–407PubMedCrossRefGoogle Scholar
  45. Tang J-Y (1996) Ultrastructural studies on sperm of Dicrocoelium chinensis (Trematoda: Digenea). Acta Zool Sin 42:225–230Google Scholar
  46. Tang J-Y, Li M-M (1996) Ultrastructural studies on spermatogenesis of Dicrocoelium chinensis (Trematoda: Digenea). Acta Zool Sin 42:225–230Google Scholar
  47. Tang J, Wang W, Wang G (1998) Studies on ultrastructure of spermatogenesis and sperm in Pseudorhipidocotyle elpichthys. Acta Hydrobiol Sin 22:168–173Google Scholar
  48. Zdarska Z, Soboleva TN, Sterba J, Valkounova J (1991) Ultrastructure of the male reproductive system of the trematode Brachylaimus aequans. Folia Parasitol 38:33–37PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Momar Talla Seck
    • 1
    Email author
  • Bernard Marchand
    • 2
  • Cheikh Tidiane Bâ
    • 3
  1. 1.Institut Sénégalais de Recherches Agricoles (ISRA)/Laboratoire National d’Elevage et de Recherches Vétérinaires (LNERV) de HannDakarSenegal
  2. 2.Laboratoire Parasites et Ecosystèmes Méditerranéens, Faculté des Sciences et TechniquesUniversité de Corse, Campus GrossettiCorteFrance
  3. 3.Laboratoire de Parasitologie-Helminthologie, Département de Biologie animale, Faculté des Sciences et TechniquesUniversité Cheikh Anta Diop de DakarDakarSenegal

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