Parasitology Research

, Volume 101, Issue 2, pp 275–280 | Cite as

Prevalence and genotyping of Giardia in husbandry systems in Taiwan

  • Bing-Mu HsuEmail author
  • Hau-Yang Wun
  • Ping-Chi Hsu
Original Paper


In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Giardia cysts. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescent microscopic examination were performed as a presumption test and confirmation test, respectively. Eight out of 107 samples analyzed by ELISA showed the presence of Giardia, among which six samples were confirmed by immunofluorescent microscopic examination, and four samples were reconfirmed by polymerase chain reaction method. Giardia species were identified by nucleic acid sequencing method of β-giardin gene. Two Giardia duodenalis assemblage A isolates and E isolates were all detected from cattle feces. This is the first identification of genotypes of G. duodenalis in the feces of feeding animals in Taiwan.


Giardiasis Fecal Specimen Polymerase Chain Reaction Mixture Giardia Cyst Polymerase Chain Reaction Template 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was funded by the National Science Council of Taiwan, R.O.C. (NSC94-2211-E-194-001).


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Earth and Environmental SciencesNational Chung Cheng UniversityTaiwanRepublic of China
  2. 2.Institute of SeismologyNational Chung Cheng UniversityTaiwanRepublic of China
  3. 3.Department of Safety, Health and Environmental EngineeringNational Kaohsiung First University of Science and TechnologyTaiwanRepublic of China

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