Prevalence and genotyping of Giardia in husbandry systems in Taiwan
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In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Giardia cysts. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescent microscopic examination were performed as a presumption test and confirmation test, respectively. Eight out of 107 samples analyzed by ELISA showed the presence of Giardia, among which six samples were confirmed by immunofluorescent microscopic examination, and four samples were reconfirmed by polymerase chain reaction method. Giardia species were identified by nucleic acid sequencing method of β-giardin gene. Two Giardia duodenalis assemblage A isolates and E isolates were all detected from cattle feces. This is the first identification of genotypes of G. duodenalis in the feces of feeding animals in Taiwan.
KeywordsGiardiasis Fecal Specimen Polymerase Chain Reaction Mixture Giardia Cyst Polymerase Chain Reaction Template
This work was funded by the National Science Council of Taiwan, R.O.C. (NSC94-2211-E-194-001).
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