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Parasitology Research

, Volume 101, Issue 2, pp 275–280 | Cite as

Prevalence and genotyping of Giardia in husbandry systems in Taiwan

  • Bing-Mu HsuEmail author
  • Hau-Yang Wun
  • Ping-Chi Hsu
Original Paper

Abstract

In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Giardia cysts. Enzyme-linked immunosorbent assay (ELISA) and immunofluorescent microscopic examination were performed as a presumption test and confirmation test, respectively. Eight out of 107 samples analyzed by ELISA showed the presence of Giardia, among which six samples were confirmed by immunofluorescent microscopic examination, and four samples were reconfirmed by polymerase chain reaction method. Giardia species were identified by nucleic acid sequencing method of β-giardin gene. Two Giardia duodenalis assemblage A isolates and E isolates were all detected from cattle feces. This is the first identification of genotypes of G. duodenalis in the feces of feeding animals in Taiwan.

Keywords

Giardiasis Fecal Specimen Polymerase Chain Reaction Mixture Giardia Cyst Polymerase Chain Reaction Template 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was funded by the National Science Council of Taiwan, R.O.C. (NSC94-2211-E-194-001).

References

  1. Adam RD (2001) Biology of Giardia lamblia. Clin Microbiol Rev 14:447–475PubMedCrossRefGoogle Scholar
  2. Appelbee AJ, Frederick LM, Heitman TL, Olson ME (2003) Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada. Vet Parasitol 112:289–294PubMedCrossRefGoogle Scholar
  3. Baker DA, Holberton DV, Marshall J (1988) Sequence of a giardin subunit cDNA from Giardia lamblia. Nucleic Acids Res 16:71–77Google Scholar
  4. Berrilli F, Di Cave D, De Liberato C, Franco A, Scaramozzino P, Orecchia P (2004) Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing. Vet Parasitol 122:193–199PubMedCrossRefGoogle Scholar
  5. Caccio SM, De Giacomo M, Pozio E (2002) Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol 32:1023–1030PubMedCrossRefGoogle Scholar
  6. Cook GC (1995) Entamoeba histolytica and Giardia lamblia infection: current diagnostic strategies. Parasite 2:107–112PubMedGoogle Scholar
  7. Faubert G (2000) Immune response to Giardia duodenalis. Clin Microbiol Rev 13:35–54PubMedCrossRefGoogle Scholar
  8. Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RCA (1997) Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol 83:44–51PubMedCrossRefGoogle Scholar
  9. Hsu BM (2003) Evaluation of analyzing methods for Giardia and Cryptosporidium in a Taiwan water treatment plant. J Parasitol 89:369–371PubMedCrossRefGoogle Scholar
  10. Hsu BM, Huang C, Hsu CLL, Hsu YF, Yeh JH (1999) Occurrence of Giardia and Cryptosporidium in the Kau-Ping river and its watershed in southern Taiwan. Water Res 33:2701–2707CrossRefGoogle Scholar
  11. Hsu BM, Huang C, Hsu YF, Hsu CLL (2000) Examination of Giardia and Cryptosporidium in water samples and fecal specimens in Taiwan. Water Sci Technol 41:87–92Google Scholar
  12. Leng X, Mosier DA, Oberst RD (1996) Simplified method for recovery and PCR detection of Cryptosporidium DNA from bovine feces. Appl Environ Microbiol 62:643–647PubMedGoogle Scholar
  13. Levine WC, Stephenson WT, Craun GF (1990) Waterborne disease outbreaks, 1986–1988. MMWR CDC Surveill Summ 39:1–13PubMedGoogle Scholar
  14. Mahbubani MH, Bej AK, Perlin MH, Schaefer III FW, Jakubowski W, Atlas RM (1992) Differentiation of Giardia duodenalis from other Giardia spp. by using polymerase chain reaction and gene probes. J Clin Microbiol 30:74–78PubMedGoogle Scholar
  15. Mayrhofer G, Andrews RH, Ey PL, Chilton NB (1995) Division of Giardia isolates into two genetically distinct assemblages by electrophoretic analysis of enzymes encoded at 27 loci and comparison with Giardia muris. Parasitology 111:11–17PubMedGoogle Scholar
  16. Monis PT, Mayrhofer G, Andrews RH, Homan WL, Limper L, Ey PL (1996) Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology 112:1–12PubMedCrossRefGoogle Scholar
  17. Monis PT, Andrews RH, Mayrhofer G, Ey PL (1999) Molecular systematics of the parasitic protozoan Giardia intestinalis. Mol Biol Evol 16:1135–1144PubMedGoogle Scholar
  18. Monis PT, Andrews RH, Mayrhofer G, Ey PL (2003) Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol 3:29–38PubMedCrossRefGoogle Scholar
  19. Morris AJ, Wilson ML, Reller LB (1992) Application of rejection criteria for stool ovum and parasite examinations. J Clin Microbiol 30:3213–3216PubMedGoogle Scholar
  20. O’Handley RM, Cockwill C, McAllister TA, Jelinski M, Morck DW, Olson ME (1999) Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhea. J Am Vet Med Assoc 214:391–396PubMedGoogle Scholar
  21. O’Handley RM, Olson ME, Fraser D, Adams P, Thompson RCA (2000) Prevalence and genotypic characterisation of Giardia in dairy calves from western Australia and western Canada. Vet Parasitol 90:193–200PubMedCrossRefGoogle Scholar
  22. Olson ME, O’Handley RM, Ralston B, Thompson RCA (2004) Emerging issues of Cryptosporidium and Giardia infections in cattle. Trends Parasitol 20:185–191PubMedCrossRefGoogle Scholar
  23. Rochelle PA, De Leon R, Stewart MH, Wolfe RL (1997) Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water. Appl Environ Microbiol 63:106–114PubMedGoogle Scholar
  24. Ruest N, Couture Y, Faubert GM, Girard C (1997) Morphological changes in the jejunum of calves naturally infected with Giardia spp. and Cryptosporidium spp. Vet Parasitol 69:177–186PubMedCrossRefGoogle Scholar
  25. Thompson RCA (2003) Molecular epidemiology of Giardia and Cryptosporidium infections. J Parasitol 89:134–140CrossRefGoogle Scholar
  26. Thompson RCA, Hopkins RM, Homan WL (2000) Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol Today 16:210–213PubMedCrossRefGoogle Scholar
  27. van Keulen H, Macechko PT, Wade S, Schaaf S, Wallis PM, Erlandsen SL (2002) Presence of human Giardia in domestic, farm, and wild animals, and environmental samples suggests a zoonotic potential for giardiasis. Vet Parasitol 108:97–107PubMedCrossRefGoogle Scholar
  28. Weiss JB, van Keulen H, Nash TE (1992) Classification of subgroups of Giardia lamblia based upon ribosomal RNA gene sequence using the polymerase chain reaction. Mol Biochem Parasitol 54:73–86PubMedCrossRefGoogle Scholar
  29. WHO (1996) The World Health Report 1996. WHOGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of Earth and Environmental SciencesNational Chung Cheng UniversityTaiwanRepublic of China
  2. 2.Institute of SeismologyNational Chung Cheng UniversityTaiwanRepublic of China
  3. 3.Department of Safety, Health and Environmental EngineeringNational Kaohsiung First University of Science and TechnologyTaiwanRepublic of China

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