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Prevalence of Fasciola hepatica (ELISA and fecal analysis) in ruminants from a semi-desert area in the northwest of Mexico

Parasitology Research volume 101, pages 127–130 (2007)Cite this article

Abstract

The aim of the present study was to estimate the prevalence of Fasciola hepatica in cattle, goats and sheep from the municipalities of Guaymas and Cajeme, Sonora State, Mexico using the indirect ELISA and Benedeck’s sedimentation tests. A total of 2,936 serum and fecal samples from 1,346 bovines, 1,199 goats and 381 sheep were analyzed. Other parameters such as breed and age were also taken into consideration. The results indicated a moderate percentage of prevalence for fasciolosis in these municipalities. In bovines, a prevalence of 11.4 ± 0.9 was found using the sedimentation test and 24.4 ± 1.2 for the indirect ELISA. In goats, a prevalence of 24.5 ± 1.2 was found for the fecal analysis and 43 ± 1.5 for the indirect ELISA. In sheep, the prevalence for both tests was 19.4 ± 2.0 and 30.6 ± 2.7, respectively. Results showed a greater proportion of positive animals when the ELISA test was used than when the Benedeck’s sedimentation test was applied. Moderate agreement was observed between both laboratory tests for the three species of ruminants included in the study (Cohen’s kappa, \( \widehat{K} \); cattle \( \widehat{K} \) = 0.527, goats \( \widehat{K} \) = 0.541 and sheep \( \widehat{K} \) = 0.539).

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Acknowledgements

The authors would like to acknowledge the CENID-PAVET-INIFAP for their permission in using their facilities to carry out the ELISA tests. This study was partially supported by the Project CONACYT-34942-B.

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Affiliations

  1. Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Antonio Caso S/n, Fracc. Villa ITSON, C.P. 85130, Cd. Obregón, Sonora, Mexico

    J. A. Munguía-Xóchihua

  2. Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootencia, Ciudad Universitaria, Universidad Nacional Autónoma de México, 04510, Distrito Federal, Mexico

    F. Ibarra-Velarde & H. Quiroz-Romero

  3. Departamento de Genética y Estadística, Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Universidad Nacional Autónoma de México, 04510, Distrito Federal, Mexico

    A. Ducoing-Watty

  4. CENID-Parasitología/INIFAP, Km. 11.5 Carretera Cuernavaca Cuautla, 62500, Morelos, Mexico

    N. Montenegro-Cristino

Authors
  1. J. A. Munguía-Xóchihua
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  2. F. Ibarra-Velarde
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  3. A. Ducoing-Watty
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  4. N. Montenegro-Cristino
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  5. H. Quiroz-Romero
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Correspondence to J. A. Munguía-Xóchihua.

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Munguía-Xóchihua, J.A., Ibarra-Velarde, F., Ducoing-Watty, A. et al. Prevalence of Fasciola hepatica (ELISA and fecal analysis) in ruminants from a semi-desert area in the northwest of Mexico. Parasitol Res 101, 127–130 (2007). https://doi.org/10.1007/s00436-006-0438-y

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  • DOI: https://doi.org/10.1007/s00436-006-0438-y

Keywords

  • Irrigation Area
  • ELISA Test
  • Indirect ELISA
  • Positive Animal
  • Fecal Analysis