Abstract
Multilocus enzyme electrophoresis (MEE) was used to genetically compare Moniezia spp. collected from sheep and cattle in southern Australia. Fifteen enzyme loci were established for the genetic characterisation of individual specimens of Moniezia, which represents a significant increase in the number of loci that was previously available for these cestodes. Another four enzyme loci were also identified as providing potential genetic markers. There were no differences in staining activity or banding patterns of different body regions of individual cestodes. Fixed genetic differences between Moniezia expansa and Moniezia benedeni were detected at 10 (77%) loci. Individuals that lacked the diagnostic morphological characters (i.e. the arrangement pattern of the interproglottidal glands) could be assigned to either morphospecies based on their MEE profiles. Genetic differences were also detected among individuals of the two morphospecies. One specimen of M. expansa had fixed genetic differences at 33% of enzyme loci when compared to all other specimens of M. expansa. Fixed genetic differences were also detected between the two groups of M. benedeni at 12 of 13 (92%) enzyme loci. This magnitude of genetic difference was greater than that detected between the two morphospecies. This provides evidence for the existence of cryptic species in the genus Moniezia.
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References
Andrews RH, Chilton NB (1999) Multilocus enzyme electrophoresis: a valuable technique for providing answers to problems in parasite systematics. Int J Parasitol 29:213–253
Andrews RH, Beveridge I, Adams M, Baverstock PR (1988) Identification of life cycle stages of the nematode Echinocephalus overstreeti by allozyme electrophoresis. J Helminthol 62:153–157
Andrews RH, Chilton NB, Beveridge IB, Spratt DM, Mayrhofer G (1992) Genetic markers for the identification of three Australian tick species at various stages of their life cycle. J Parasitol 78:366–368
Ba T, Wang XQ, Renaud F, Euzet L, Marchand B, De Meeüs T (1993) Diversity and specificity in cestodes of the genus Moniezia; genetic evidence. Int J Parasitol 23:853–857
Balasubramanian MP, Nellaiappan K, Ramalingam K (1984) Changes in isozyme pattern of alkaline phosphatase from Moniezia benedeni in relation to growth and differentiation of the proglottids. Riv Parassitol XLV:45–48
Baverstock PR, Adams M, Beveridge I (1985) Biochemical differentiation in bile duct cestodes and their marsupial hosts. Mol Biol Evol 2:321–337
Beveridge I (1994) 17. Family Anoplocephalidae Cholodkovsky, 1902. In: Khalil LF, Jones A, Bray RA (eds) Keys to the cestode parasites of vertebrates. CAB International, Wallingford, pp 315–366
Dhandayuthapani S, Nellaiappan K, Balasubramanian MP, Ramalingam K (1984) Isozyme pattern of lactate and malate dehydrogenases of Moniezia benedeni. Riv Parassitol XLV:459–463
Dorchies P (1999) Moniezia expansa: importance du parasitisme, conséquences économiques. Rev Méd Vét 150:107–110
Ershov VS (1956) Parasitology and parasitic diseases of livestock. State Publishing House for Agricultural Literature, Moscow (English translation: Jerusalem, Israel Program for Scientific Translations, 1960)
Hermoso R, Valero A, Monteoliva M (1982) Estudio de isoenzimas y proteinas solubles en Moniezia expansa Rudolphi (1810) y Avitellina centripunctata Rivolta (1874), por electroforesis y electroenfoque. Rev Iber Parasitol 42:109–116
Hu M, Gasser RB, Chilton NB, Beveridge I (2005) Genetic variation in the mitochondrial cytochrome c subunit 1 within three species of Progamotaenia (Cestoda: Anoplocephalidae) from macropodid marsupials. Parasitology 130:117–129
Johnson MR, Hoberg EP (1989) Differentiation of Moniezia expansa and Moniezia benedeni (Eucestoda: Cyclophyllidea) by isoelectric focusing. Can J Zool 67:1471–1495
Juyal PD (1987) How serious are Moniezia infections in domestic ruminants? Livest Advis 12:47–48
Khera S, Arora S (1984) Histochemical studies of certain enzymes in interproglottidal glands of Moniezia expansa. Ind J Parasitol 8:281–283
Norman RJdeB, Chilton NB (1994) An electrophoretic comparison of Physaloptera Rudolphi, 1819 (Nematoda: Physalopteridae) from two species of Australian bandicoot (Marsupialia: Peramelidae). Syst Parasitol 29:223–228
Pritchard MH, Kruse GOW (1982) The collection and preservation of animal parasites. University of Nebraska Press, Nebraska
Richardson BJ, Baverstock PR, Adams M (1986) Allozyme electrophoresis: a handbook for animal systematics and population studies. Academic Press, Sydney
Sneath PHRA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman, San Francisco, CA
Spasskii AA (1951) Essentials of cestodology, vol 1: anoplocephalate tapeworms of domestic and wild animals. Academy of Sciences of the USSR, Moscow (English translation: Jerusalem, Israel Program for Scientific Translations, 1963)
Spratt DM, Beveridge I, Walter EL (1991) A catalogue of Australasian monotremes and marsupials and their recorded helminth parasites. Rec S Aust Mus Monogr Ser 1:1–105
Taylor EL (1928) Moniezia a genus of cestode worms, and the proposed reduction of its species to three. Proc US Natl Mus (Wash) 74:1–9
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Chilton, N.B., O’Callaghan, M.G., Beveridge, I. et al. Genetic markers to distinguish Moniezia expansa from M. benedeni (Cestoda: Anoplocephalidae) and evidence of the existence of cryptic species in Australia. Parasitol Res 100, 1187–1192 (2007). https://doi.org/10.1007/s00436-006-0388-4
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DOI: https://doi.org/10.1007/s00436-006-0388-4