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Vaccination of buffaloes with Fasciola gigantica recombinant fatty acid binding protein

Parasitology Research volume 97, pages 129–135 (2005)Cite this article

Abstract

Recombinant fatty acid binding protein (rFABP) of Fasciola gigantica was expressed in Escherichia coli and used as vaccine in Freund’s adjuvant to evaluate the level of protection induced in buffalo (Bubalus bubalis) calves. Fifteen buffalo calves were distributed to three groups of five calves each. An antigen dose of 400 μg for each of the three immunizations at 3-week intervals, and a challenge dose of 600 metacercariae was administered per calf. Levels of anti-FABP antibodies increased rapidly by 2 weeks after the first immunization and were always significantly higher in the immunized-challenged group than in the infected control group. Immunization with FABP induced both humoral and cell-mediated immune response in these animals. Vaccination showed a moderate level of protection in terms of reduced fluke burden (35.8%) and liver damage as assayed by aspartate aminotransferase and sulfhydryl group levels as well as anti-fecundity effect of the vaccine.

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References

  • Agricultural Research Data Book (2002) Indian Agricultural statistics Research Institute. New Delhi, p 28

  • Backer PE, Knoblock KF (1982) Bovine co-stimulator II: generation and maintenance of co-stimulator dependent bovine lymphoblastoid cell lines. Vet Immunol Immunopathol 2:467–469

    Google Scholar 

  • Banerjee GC (1991) A textbook of animal husbandry, 7th edn. Mohan Primlani for Oxford and IBH pub, pp 112–114

  • Bounous DI, Campagndi RP, Brown J (1992) Comparison of MTT colorimetric assay and tritiated thymidine uptake for lymphocyte proliferation assay using chicken spleenocytes. Adv Dis 36:1022–1027

    CAS  Google Scholar 

  • Estuningsih ES, Smooker PM, Wiedosari E, Widjajanti S, Vaiano A, Partotomo S, Spithill TW (1997) Evaluation of antigens of Fasciola gigantica as vaccine against tropical fasciolosis. Int J Parasitol 11:1419–1428

    Google Scholar 

  • FAO (1994) Diseases of domestic animals caused by flukes. Food and Agriculture Organization of the United Nations, Rome, p 49

  • Hillyer GV (1985) Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined antigen. Am J Trop Med Hyg 34:1127–1131

    CAS  PubMed  Google Scholar 

  • Hillyer GV, Haroun EM, Hermandez A, De Galanes MS (1987) Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen. Am J Trop Med Hyg 37:363–369

    CAS  PubMed  Google Scholar 

  • Kearl LC (1982) Nutrient requirements of ruminants in developing countries. International Feedstuffs Institute, Utah Agricultural Experiment Station, Utah State University, Logan

  • Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:630–684

    Google Scholar 

  • Lowry OH, Rosenberg NJ, Farr AM, Randell RJ (1951) Protein measurement with folin reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Mehra UR, Dass RS, Verma AK, Sharma RL, Yadav SC (1999) Effect of Fasciola gigantica infection on growth and nutrient utilization in buffalo calves. Vet Rec 145:699–702

    CAS  PubMed  Google Scholar 

  • Meyer F, Meyer H, Bueding E (1970) Lipid metabolism in the parasitic and free living flatworms, Schistosoma mansoni and Dugesia dorotocephala. Biochem Biophys Acta 210:257–266

    CAS  PubMed  Google Scholar 

  • Muro A, Ramajo V, Lopez J, Simon F, Hillyer GV (1997) Fasciola hepatica: vaccination of rabbits with recombinant and native antigens related to fatty acid binding proteins. Vet Parasitol 69:219–229

    Article  CAS  PubMed  Google Scholar 

  • Ockner RK (1990) Historic overviews of the studies on fatty acid binding proteins. Mol Cell Biochem 98:3–9

    Article  CAS  PubMed  Google Scholar 

  • Peterson JD, Herzenberg LA, Vasquez K, Waltenbaugh C (1998) Glutathione levels in the antigen presenting cells modulate Th1 versus Th2 response patterns. Proc Natl Acad Sci USA 95:3071–3076

    Article  CAS  PubMed  Google Scholar 

  • Ramajo V, Oleaga A, Casanueva P, Hillyer GV, Muro A (2001) Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins. Vet Parasitol 97:35–46

    Article  CAS  PubMed  Google Scholar 

  • Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63

    CAS  PubMed  Google Scholar 

  • Sanyal PK (2001) Control of tropical fasciolosis in cattle and buffaloes in India at the backdrop of its integrated management. J Vet Parasitol 15:13–16

    Google Scholar 

  • Sedlack J, Lindsay RH (1968) Estimation of total, protein bound, and nonprotein bound sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205

    Article  PubMed  Google Scholar 

  • Sriveny D, Raina OK, Verma J, Singh BP, Yadav SC (2002) Purification of Fasciola gigantica recombinant fatty acid binding protein. J Vet Parasitol 16:39–41

    Google Scholar 

  • Sriveny D, Raina OK, Verma J, Yadav SC (2003) Expression of Fasciola gigantica recombinant fatty acid binding protein. Ind J Biotech 2:549–557

    Google Scholar 

  • Tendler M, Brito CA, Vilar MM, Serra-Freire N, Diogo CM, Almeida MS, Delbem AC, De Silva JF, Sanivo W, Garratt RC, Simpson AJ (1996) A Schistosoma mansoni fatty acid binding protein Sm14 is the potential basis of dual purpose anti-helminth vaccine. Proc Natl Acad Sci USA 93:269–273

    Article  CAS  PubMed  Google Scholar 

  • Towbin H, Stachelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350

    CAS  PubMed  Google Scholar 

  • Yadav SC, Sharma RL, Kalicharan A, Mehra UR, Das RS, Verma AK (1999) Primary experimental infection of riverine buffaloes with Fasciola gigantica. Vet Parasitol 82:285–296

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors are thankful to the authorities of the National Agricultural Technology Project (CGP) for financial assistance to undertake this study. The first author is also thankful to IVRI for providing JRF during his MVSc programme.

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Affiliations

  1. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122, UP, India

    P. Azhahia Nambi, S. C. Yadav, O. K. Raina & D. Sriveny

  2. Division of Biochemistry and Food sciences, Indian Veterinary Research Institute, Izatnagar, 243122, UP, India

    Mohini Saini

Authors
  1. P. Azhahia Nambi
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  2. S. C. Yadav
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  3. O. K. Raina
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  4. D. Sriveny
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  5. Mohini Saini
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Corresponding author

Correspondence to O. K. Raina.

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Declaration: All the experiments conducted on the buffalo calves were as per the guidelines laid down by the Animal Ethics Committee of Indian Veterinary Research Institute, Izatnagar, Bareilly, India.

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Nambi, P.A., Yadav, S.C., Raina, O.K. et al. Vaccination of buffaloes with Fasciola gigantica recombinant fatty acid binding protein. Parasitol Res 97, 129–135 (2005). https://doi.org/10.1007/s00436-005-1397-4

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  • DOI: https://doi.org/10.1007/s00436-005-1397-4

Keywords

  • Infected Control
  • Fatty Acid Binding Protein
  • Worm Burden
  • Challenge Infection
  • Vaccination Trial