Abstract
The cell-mediated response in BALB/c mice infected either by Trichinella pseudospiralis or Trichinella spiralis was compared at days 30–50 post-infection (muscle phase). The former species is non-encapsulated, whereas the latter is encapsulated in host muscles. The pattern of response against the two species was similar. Both species elicited TH0 or TH1/TH2 response, with the last one being dominant. Productions of interferon gamma (IFN-γ), interleukin (IL)-4 and IL-5 were observed after antigenic restimulation of splenocytes from infected mice. No significant difference was observed between the levels of response to concanavalin A (Con-A) by the splenocytes from both infected and non-infected animals. There was a significant increase in serum IgG1 and IgG2a. Flow cytometric analysis revealed a marked proliferative response of splenocytes from infected mice to worm antigens, dominated by B (CD19) lymphoblasts. Only a few helper (CD4+) and cytotoxic (CD8+) T lymphoblasts were present. This was confirmed by an up-regulation of CD69, with a dominant expression on B lymphoblasts. In conclusion, the minimal or lack of intense cellular response against T. pseudospiralis in muscles is likely not due to depression of cell-mediated immunity.
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Acknowledgements
This study was supported by a research grant from the University of Hong Kong to R.C.K. We are grateful to the invaluable assistance of Mr. W.H. Sit, Ms. H.M. Lun and Ms. Y.Y.Y. Chung.
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Lee, K.M., Ko, R.C. Cell-mediated response at the muscle phase of Trichinella pseudospiralis and Trichinella spiralis infections. Parasitol Res 99, 70–77 (2006). https://doi.org/10.1007/s00436-005-0101-z
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DOI: https://doi.org/10.1007/s00436-005-0101-z