Abstract
Trichinella spiralis and Trichinella nativa are both common wildlife parasites in Finland. However, they differ substantially in their resistance to below 0°C temperatures in their natural hosts. T. nativa can live in frozen fox meat for years, whereas T. spiralis dies when frozen. In mouse muscle, the difference is not as evident; even T. nativa cannot maintain infectivity when kept at −20°C for 1 week. Crude larval protein extracts of these two parasite species were analyzed by two-dimensional gel electrophoresis (2DE). The protein patterns showed clear differences, but matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprint followed by database searches failed to identify these proteins, suggesting that they may still be uncharacterized. The patterns compared after freezing treatment at −20°C revealed changes in the intensity of some protein spots. The antigenic differences of the species were analyzed with two-dimensional Western blots, which showed T. spiralis-specific proteins.
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Acknowledgements
We thank Dr. Nisse Kalkkinen and Gunilla Rönnholm for the expertise concerning MALDI-TOF and Docent Pekka Varmanen for helping with PDQuest software. The study was supported by the Finnish Veterinary Foundation. The authors declare that the experiments comply with the current laws in Finland.
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Näreaho, A., Ravanko, K., Hölttä, E. et al. Comparative analysis of Trichinella spiralis and Trichinella nativa proteins by two-dimensional gel electrophoresis. Parasitol Res 98, 349–354 (2006). https://doi.org/10.1007/s00436-005-0086-7
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DOI: https://doi.org/10.1007/s00436-005-0086-7