Parasitology Research

, Volume 98, Issue 6, pp 557–560

Oregano (Lippia spp.) kills Giardia intestinalis trophozoites in vitro: antigiardiasic activity and ultrastructural damage

  • Martha Ponce-Macotela
  • Yadira Rufino-González
  • Angélica González-Maciel
  • Rafael Reynoso-Robles
  • Mario Noé Martínez-Gordillo
Original Paper

Abstract

In the world, giardiosis is still a very important parasitic disease; only in Asia, Africa and America, there are more than 200 million of infected people in a year. The usual treatments are drugs that produce undesirable secondary effects, perhaps favouring the resistant strain selection. One alternative is to research compounds from plants used as antidiarrhoeic or antiparasitic in the traditional medicine. In a previous work, we found that Lippia beriandieri (Oregano) revealed to be more potent than tinidazole, a common antigiardiasic drug. In this current work, we tested the cell viability by re-culture and reduction of MTT-tetrazolium salts to MTT-formazan, and we showed the effect of oregano ethanolic extracts against Giardia intestinalis (synonyms: Giardia duodenalis, Giardia lamblia) trophozoites at concentrations ranging form 58 to 588 μg. We demonstrated the ultrastructural injury produced by oregano extracts in this parasite. Trophozoites lost their size and shape and showed damage in nucleus structure, perhaps by breaking the pattern of nucleoskeleton proteins.

References

  1. Adam RD (2001) Biology of Giardia lamblia. Clin Microbiol Rev 14:447–475CrossRefPubMedGoogle Scholar
  2. Astiazarán-García H, Espinosa-Cantellano M, Castañón G, Chávez-Munguía V, Martínez-Palomo A (2000) Giardia lamblia: effect of infection with symptomatic and asymptomatic isolates on the growth of gerbils (Meriones unguiculatus). Exp Parasitol 95:128–135PubMedGoogle Scholar
  3. Bulut BU, Gulnar SB, Syseb D (1996) Alternative treatment protocols in giardiasis: a pilot study. Scand J Infect Dis 28:493–495PubMedGoogle Scholar
  4. Comité OMS d’Experts (1988) Importance des parasitoses intestinalis en santé publique. Bull Org Mon San 66:23–34Google Scholar
  5. Freeman CD, Klutman NE, Lamp KC (1997) Metronidazole a therapeutic review and update. Drugs 54:679–708PubMedCrossRefGoogle Scholar
  6. Gardner TB, Hill D (2001) Treatment of giardiasis. Clin Microbiol Rev 14:114–128CrossRefPubMedGoogle Scholar
  7. Gillis GC, Weisman LR (1996) Secnidazole. A review of its antimicrobial activity, pharmacokinetic properties and therapeutic use en the management of protozoal infections and bacterial vaginosis. Drugs 51:621–638PubMedGoogle Scholar
  8. Harris JC, Plumber S, Lloyd D (2001) Antigiardial drugs. Appl Microbiol Biotechnol 57:614–619CrossRefPubMedGoogle Scholar
  9. Johnson PJ (1993) Metronidazole and drug resistance. Parasitol Today 9:183–186CrossRefPubMedGoogle Scholar
  10. Lasky AL (2000) The nucleoskeleton: go EAST, young man. Nat Cell Biol 2:241–243CrossRefPubMedGoogle Scholar
  11. Lemée V, Zaharia I, Nevez G, Rabodonirina M, Brasseur P, Ballet JJ, Favennec L (2000) Metronidazole and albendazole susceptibility of 11 clinical isolates of Giardia duodenalis from France. J Antimicrob Chemother 46:819–821CrossRefPubMedGoogle Scholar
  12. Martinez V, Caumes E (2001) Métronidazole. Ann Dermatol Venereol 128:903–909PubMedGoogle Scholar
  13. Mendelson RM (1980) The treatment of giardiasis. Trans R Soc Trop Med Hyg 74:438–439CrossRefPubMedGoogle Scholar
  14. Pickering LK (1985) Problems in diagnosing and managing giardiasis. Pediatr Infect Dis 4(3 Suppl) S6–S10PubMedGoogle Scholar
  15. Ponce-Macotela M, Navarro-Alegría I, Martínez-Gordillo MN, Álvarez-Chacón R (1994) Efecto antigiardiásico in vitro de 14 extractos de plantas. Rev Invest Clin 46:343–347PubMedGoogle Scholar
  16. Ponce-Macotela M, Gómez-Garduño J, González-Maciel A, Reynoso-Robles R, Anislado-Tolentino V, Martínez-Gordillo MN (2001a) Determinación in vitro de la susceptibilidad a la nitazoxanida de cuatro aislados de Giardia intestinalis obtenidos de diferentes huéspedes. Rev Invest Clin 53:41–45PubMedGoogle Scholar
  17. Ponce-Macotela M, Rufino-González Y, de la Mora-de la Mora JI, González-Maciel A, Reynoso-Robles R, Martínez-Gordillo MN (2001b) Mortality and morphological changes in Giardia duodenalis induced by exposure to ethanolic extracts of Justicia spicigera. Proc West Pharmacol Soc 44:151–152PubMedGoogle Scholar
  18. Ponce-Macotela M, Martínez-Gordillo MN, Bermúdez-Cruz RM, Salazar-Schettino PM, Ortega-Pierres G, Ey PL (2002) Unusual prevalence of the Giardia intestinalis A-II subtype amongst isolates from humans and domestic animals in Mexico. Int J Parasitol 32:1201–1202CrossRefPubMedGoogle Scholar
  19. Romero CR, Robert GL, Muñoz GMR, Geyne CA (1997) Nitazoxanide for the treatment of intestinal protozoan and helminthic infections in Mexico. Trans R Soc Trop Med Hyg 91:701–703CrossRefPubMedGoogle Scholar
  20. Sangster N, Batterham P, Chapman DH, Duraisingh M, Jambre LL, Shirley M, Upcroft JA, Upcroft P (2002) Resistance to antiparasitic drugs: the role of molecular diagnosis. Int J Parasitol 32:637–652CrossRefPubMedGoogle Scholar
  21. Towson SM, Boreham PFL, Upcroft P, Upcroft JA (1994) Resistance to the nitroheterocyclic drugs. Acta Trop 56:173–194CrossRefPubMedGoogle Scholar
  22. Upcroft P (1994) Multiple drug resistance in the pathogenic protozoa. Acta Trop 56:195–212CrossRefPubMedGoogle Scholar
  23. Upcroft P, Upcroft JA (2001) Drug targets and mechanisms of resistance in the anaerobic protozoa. Clin Microbiol Rev 14:150–164CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Martha Ponce-Macotela
    • 1
  • Yadira Rufino-González
    • 1
  • Angélica González-Maciel
    • 2
  • Rafael Reynoso-Robles
    • 2
  • Mario Noé Martínez-Gordillo
    • 1
  1. 1.Parasitología ExperimentalInstituto Nacional de Pediatría, Insurgentes SurMéxicoMexico
  2. 2.Microscopia Electrónica del Instituto Nacional de PediatríaMéxicoMexico

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