Oregano (Lippia spp.) kills Giardia intestinalis trophozoites in vitro: antigiardiasic activity and ultrastructural damage
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In the world, giardiosis is still a very important parasitic disease; only in Asia, Africa and America, there are more than 200 million of infected people in a year. The usual treatments are drugs that produce undesirable secondary effects, perhaps favouring the resistant strain selection. One alternative is to research compounds from plants used as antidiarrhoeic or antiparasitic in the traditional medicine. In a previous work, we found that Lippia beriandieri (Oregano) revealed to be more potent than tinidazole, a common antigiardiasic drug. In this current work, we tested the cell viability by re-culture and reduction of MTT-tetrazolium salts to MTT-formazan, and we showed the effect of oregano ethanolic extracts against Giardia intestinalis (synonyms: Giardia duodenalis, Giardia lamblia) trophozoites at concentrations ranging form 58 to 588 μg. We demonstrated the ultrastructural injury produced by oregano extracts in this parasite. Trophozoites lost their size and shape and showed damage in nucleus structure, perhaps by breaking the pattern of nucleoskeleton proteins.
- Comité OMS d’Experts (1988) Importance des parasitoses intestinalis en santé publique. Bull Org Mon San 66:23–34Google Scholar
- Ponce-Macotela M, Gómez-Garduño J, González-Maciel A, Reynoso-Robles R, Anislado-Tolentino V, Martínez-Gordillo MN (2001a) Determinación in vitro de la susceptibilidad a la nitazoxanida de cuatro aislados de Giardia intestinalis obtenidos de diferentes huéspedes. Rev Invest Clin 53:41–45PubMedGoogle Scholar
- Ponce-Macotela M, Rufino-González Y, de la Mora-de la Mora JI, González-Maciel A, Reynoso-Robles R, Martínez-Gordillo MN (2001b) Mortality and morphological changes in Giardia duodenalis induced by exposure to ethanolic extracts of Justicia spicigera. Proc West Pharmacol Soc 44:151–152PubMedGoogle Scholar