Skip to main content
SpringerLink
Log in

Antibodies against Leishmania cross-react with Crithidia luciliae: indirect immunofluorescence and Dot-ELISA study in dogs

  • Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Leismaniasis is zoonotic parasitic disease distributed in many areas of Mediterranean basin. Because of its pathogenicity and difficulty to grow in vitro, we used Leishmania infantum as primary and Crithidia luciliae as secondary source of antigen. We compared 100 canine sera by indirect immunofluorescence and Dot-ELISA. Both atigens showed same results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Herwaldt BL (1999) Leishmaniasis. Lancet 354:1191–1199

    Article  PubMed  CAS  Google Scholar 

  • Herwaldt BL (2001) Laboratory-acquired parasite infections from accidental exposures. Clin Microbiol Rev 14(4):659–688

    Article  PubMed  CAS  Google Scholar 

  • Howard MK, Pharaoh MM, Ashall F, Miles MA (1991) Human urine stimulates growth of Leishmania in vitro. Trans R Soc Trop Med Hyg 85:477–479

    Article  PubMed  CAS  Google Scholar 

  • Lopez-Brea M (1983) Diagnostico serologico de la leishmaniasis vesceral por imunofluorescencia indirecta. Rev Clin Esp 169(4):233–235

    PubMed  CAS  Google Scholar 

  • Limoncu EM, Balcioglu IC, Yereli C, Ozbel Y, Ozbilgin A (1997) A new experimental in vitro culture medium for cultivation of Leishmania species. J Clin Microbiol 35(9):2430–2431

    PubMed  CAS  Google Scholar 

  • Mancianti F, Pedonese F, Poli A (1996) Evaluation of dot enzyme-linked immunosorbent assay (dot-ELISA) for the serodiagnosis of canine leishmaniosis as compared with indirect immunofluorescence assay. Vet Parasitol 65:1–9

    Article  PubMed  CAS  Google Scholar 

  • Monteon VM, Guzman-Rojas L, Negrete-Garcia C, Rosales-Encina JL, Lopez PAR (1997) Serodiagnosis of American trypanosomosis by using nonpathogenic trypanosomatid antigen. J Clin Microbiol 35(12):3316–3319

    PubMed  CAS  Google Scholar 

  • Pappas GM, Hajkowski R, Hockmeyer WT (1983) Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniosis. J Immunol Methods 64:204–214

    Article  Google Scholar 

  • Seghal S, Aikat BK, Pathania AGS (1982) Immune complexes in Indian kala-azar. Bull W H O 60(6):945–949

    Google Scholar 

  • Vercammen F, Berkvens D, Brandt J, Vansteenkiste W (1998) A sensitive and specific 30-min Dot-ELISA for the detection of anti-Leishmania antibodies in the dog. Vet Parasitol 79:221–228

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

Authors are grateful to Prof. Heinz Mehlhorn for all his help and support. Also we thank to Iva Stimac for all her help and support.

Author information

Authors and Affiliations

  1. Department of Parasitology and Parasitic Diseases, Veterinary Faculty, University of Zagreb, Heinzelova 55, 10000, Zagreb, Croatia

    Franjo Martinkovic & Albert Marinculic

Authors
  1. Franjo Martinkovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Albert Marinculic
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martinkovic, F., Marinculic, A. Antibodies against Leishmania cross-react with Crithidia luciliae: indirect immunofluorescence and Dot-ELISA study in dogs. Parasitol Res 98, 378–380 (2006). https://doi.org/10.1007/s00436-005-0072-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-005-0072-0

Keywords

Search

Navigation