Abstract
Copper/zinc (Cu/Zn) superoxide dismutase (SOD) activity was identified for the first time in both crude somatic extracts (CE) and excretory/secretory (E/S) products of Trichinella pseudospiralis. It was the dominant SOD in infective-stage larvae. Native polyacrylamide gel electrophoresis of CE and E/S products yielded a prominent band, which was cyanide-sensitive and was partly inhibited by hydrogen peroxide in SOD assay. Cytosolic Cu/Zn SOD was cloned. The 471-bp full-length cDNA sequence contained an open reading frame of 157 amino acids. The gene contained three introns. Quantitative reverse transcription–polymerase chain reaction indicated that the expression of cytosolic Cu/Zn SOD was substantially higher in infective-stage larvae than in adult worms. Cluster analysis showed that the sequence of the Cu/Zn SOD of T. pseudospiralis, an adenophorean nematode, is related to those of Brugia pahangi, Acanthocheilonema viteae, Onchocerca volvulus, and Haemonchus contortus (all belonging to the sercenentean group).
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We are grateful for the invaluable assistance of Ms. Y.Y.Y. Chung and H.M. Lun.
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The work was supported by a grant from the Hong Kong Research Grant Council (HKU 426/96M) to R.C.K. and by grants from the University of Hong Kong Research Committee to both R.C.K. and C.H.M.
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Wu, W.K., Mak, C.H. & Ko, R.C. Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis . Parasitol Res 98, 281–287 (2006). https://doi.org/10.1007/s00436-005-0056-0
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DOI: https://doi.org/10.1007/s00436-005-0056-0