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Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms

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Abstract

The genetic variability of 61 Trypanosoma cruzi isolates from 47 chronic chagasic patients of Minas Gerais state was analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) using M13-40, λgt11-F, and L15996 primers. Cluster analysis by unweighted pair group method analysis was applied to RAPD profiles, and cluster analysis used to verify a possible correlation among different clinical forms of the disease from these patients. The T. cruzi isolates showed distinct grouping on tree topology, with the isolates not being possible to establish a correlation to the clinical forms of Chagas’ disease. These data showed that the T. cruzi isolates from these patients would compose a group of populations well correlated genetically.

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Acknowledgements

This work was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Edital Universal/2001), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), and a master fellowship of the FAPEMIG. We thank Afonso Costa Viana and Orlando Carlos Magno from the Departamento de Parasitologia, and Jamil Silvano Oliveira from the Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG for technical assistance.

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Authors and Affiliations

  1. Laboratório de Biologia do Trypanosoma cruzi e doença de Chagas, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 Caixa Postal 486, 31270-901, Belo Horizonte, Minas Gerais, Brazil

    D. A. D’Ávila, W. S. F. Meira, E. Chiari & L. M. C. Galvão

  2. Departamento de Medicina Preventiva e Social, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    E. D. Gontijo

  3. Departamento de Ciências Biológicas, Faculdade de Medicina do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil

    E. Lages-Silva

Authors
  1. D. A. D’Ávila
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  2. E. D. Gontijo
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  3. E. Lages-Silva
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  4. W. S. F. Meira
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  5. E. Chiari
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  6. L. M. C. Galvão
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Correspondence to L. M. C. Galvão.

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D’Ávila, D.A., Gontijo, E.D., Lages-Silva, E. et al. Random amplified polymorphic DNA profiles of Trypanosoma cruzi isolates from chagasic patients with different clinical forms. Parasitol Res 98, 455–461 (2006). https://doi.org/10.1007/s00436-005-0043-5

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  • DOI: https://doi.org/10.1007/s00436-005-0043-5

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