Abstract
The study of purified alkaline phosphatase and crude extract antigen immunogenicity from Echinococcus multilocularis was carried out on BALB/c mice. The animals were immunized, then infected with E. multilocularis metacestode. The immune response against purified alkaline phosphatase was studied. Flow cytometry analysis of the CD4+ and CD8+ lymphocyte populations showed a predominance of CD4+ populations in infected immunized mice. The specific humoral response to purified alkaline phosphatase was analyzed by enzyme-linked immunosorbent assay method. We noted a stimulation of an immunoglobulin IgG response. The isotypic profile showed a prevalence of IgG1 and IgG3 in immunized infected mice compared to IgG2a and IgG2b. In addition, analysis of the profiles of the in vitro secreted cytokines, after stimulation of the splenocytes from immunized mice, was performed. The cytokine profile was a mix of Th1/Th2 types in the infected and uninfected immunized mice. The results of this study suggest a humoral mixed Th1/Th2 response, with a high predominance of Th2 response. A similar study was conducted in mice immunized with crude total antigen. The comparison of the immune response showed an important immune response in mice immunized with purified alkaline phosphatase compared to mice immunized with the crude total antigen.
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Argiro L, Henri S, Dessein H, Kouriba B, Dessein AJ, Bourgois A (2000a) Induction of a protection against S. mansoni with a MAP containing epitopes of Sm37-GAPDH and Sm10-DLC. Effect of coadsorption with GM-CSF on alum. Vaccine 3:2033–2038
Argiro L, Kohlstadt S, Henri S, Dessein H, Matabiau V, Paris P, Bourgois A, Dessein AJ (2000b) Identification of a candidate vaccine peptide on the 37 kDa Schistosoma mansoni GAPDH. Vaccine 3:2039–2048
Bauder B, Auer H, Schilcher F, Gabler C, Romig T, Bilger B, Aspock H (1999) Experimental investigations on the B and T cell immune response in primary alveolar echinococcosis. Parasite Immunol 21:409–421
Chow C, Gauci CG, Cowman AF, Lightowlers MW (2001) A gene family expressing a host-protective antigen of Echinococcus granulosus. Mol Biochem Parasitol 118:83–88
Daeki AO, Craig PS, Shambesh MK (2000) IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Ann Trop Med Parasitol 94: 319–328
Delabre-Defayolle, Sarciron ME, Audin P, Gabrion C, Duriez, Paris J, Petavy AF (1989) Echinococcus multilocularis metacestodes: biochemical and ultrastructural investigations on the effect of isatin (2-3 indoline dione) in vivo. J Antimicrob Chemother 23:237–245
Eckert J, Deplazes P (2004) Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 17:107–135
Finkelman FD, Holmes J, Katona IM, Urban JF, Beckmann MP, Park LS, Schooley KA, Coffman RL, Mosmann TR, Paul WE (1990) Lymphokine control of in vivo immunoglobulin isotype selection. Annu Rev Immunol 8:303–333
Goudot-Crozel V, Caillol D, Djabali M, Dessein AJ (1989) The major parasite surface antigen associated with human resistance to schistosomiasis is a 37-kD glyceraldehyde-3P-dehydrogenase. J Exp Med 170:2065–2080
Horton J (2002) Albendazole: a broad spectrum anthelminthic for treatment of individuals and populations. Curr Opin Infect Dis 15:599–608
Jankovic D, Sher A, Yap G (2001) Th1/Th2 effector choice in parasitic infection: decision making by committee. Curr Opin Immunol 13:403–409
Muller-Schollenberger V, Beyer W, Schnitzler P, Merckelbach A, Roth S, Kalinna BH, Lucius R (2001) Immunisation with Salmonella typhimurium-delivered glyceraldehyde-3-phosphate dehydrogenase protects mice against challenge infection with Echinococcus multilocularis eggs. Int J Parasitol 31:1441–1449
Ortona E, Rigano R, Buttari B, Delunardo F, Ioppolo S, Margutti P, Profumo E, Teggi A, Vaccari S, Siracusano A (2003) An update on immunodiagnosis of cystic echinococcosis. Acta Trop 85:165–171
Sabatini DD, Bensh K, Barnett RJ (1963) Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol 17:19–58
Sarciron ME, Bresson-Hadni S, Mercier M, Lawton P, Duranton C, Lenys D, Petavy AF, Vuitton DA (1997) Antibodies against Echinococcus multilocularis alkaline phosphatase as markers for the specific diagnosis and the serological monitoring of alveolar echinococcosis. Parasite Immunol 19:61–68
Sarciron ME, Hamoud W, Azzar G, Petavy AF (1991) Alkaline phosphatase from Echinococcus multilocularis: purification and characterization. Comp Biochem Physiol B 100:253–258
Sher A, Coffman RL (1992) Regulation of immunity to parasites by T cells and T cell-derived cytokines. Annu Rev Immunol 10:385–409
Siles-Lucas M, Merli M, Mackenstedt U, Gottstein B (2003) The Echinococcus multilocularis 14-3-3 protein protects mice against primary but not secondary alveolar echinococcosis. Vaccine 21:431–439
Stettler M, Fink R, Walker M, Gottstein B, Geary TG, Rossignol JF, Hemphill A (2003) In vitro parasiticidal effect of Nitazoxanide against Echinococcus multilocularis metacestodes. Antimicrob Agents Chemother 47:467–474
Vuitton DA (2003) The ambiguous role of immunity in echinococcosis: protection of the host or of the parasite? Acta Trop 85:119–132
Walchshofer N, Sarciron ME, Arsac C, Walbaum S, Paris J, Pétavy AF (1993) Biological effects of a dipeptide methyl ester on Echinococcus multilocularis metacestodes in vivo. Int J Pharm 100:271–277
Acknowledgements
The authors thank Centre Commun d’Imagerie Laennec (CeCIL IFR 62), and Simone Peyrol for technical support. The authors declare that the experiments comply with the current laws of the country in which the experiments were performed.
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Issaadi, N., Fraize, M., Azzouz, S. et al. Echinococcus multilocularis: immunity response to purified alkaline phosphatase in BALB/c mice. Parasitol Res 98, 218–226 (2006). https://doi.org/10.1007/s00436-005-0041-7
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DOI: https://doi.org/10.1007/s00436-005-0041-7