Abstract
Amongst the alternative strategies to the use of anthelmintics, the administration of Duddingtonia flagrans spores has already proved its efficacy in reducing the number of developing larvae of several nematode species in goat faeces. In this trial, the efficacy of this fungus against the larvae of the three major nematode species of goats was compared in various conditions of coproculture. Twelve strongyle free goats were experimentally infected with either Trichostrongylus colubriformis, Teladorsagia circumcincta or Haemonchus contortus larvae. Half of the animals received an oral dose of 5×105 Duddingtonia chlamydospores/kg BW daily for 27 days, whereas the remaining was kept as control goats. From the 7th day of administration onwards, individual coproscopical examinations as well as coprocultures, which were incubated 4, 7, 10 or 14 days at 21 or 28°C, were performed. The reduction in developing larvae due to the activity of Duddingtonia ranged from 62.8 to 99.5% compared to control. The trapping efficacy depended on temperature (better activity of the fungus at 21 than at 28°C) and on duration (larval reductions lower after 4 days than after 7, 10 and 14 days of coproculture). Teladorsagia larvae were the least trapped, and Haemonchus larvae were the most trapped.
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References
Andersen FL, Levine ND, Boatman PA (1970) Survival of third-stage Trichostrongylus colubriformis larvae on pasture. J Parasitol 56:209–232
Aumont G, Coulaud G, Grude A, Gruner L (1989) Pasture populations of cattle nematode larvae in Guadeloupe (French West Indies). Int J Parasitol 19:547–554
Barger IA, Lewis RJ, Brown GF (1984) Survival of infective larvae of nematode parasites of cattle during drought. Vet Parasitol 14:143–152
Barger IA, Siale K, Banks DJD, Le Jambre LF (1994) Rotational grazing for control of gastrointestinal nematodes of goats in a wet tropical environment. Vet Parasitol 53:109–116
Barron GL (1977) The nematode-destroying fungi. Topics in Mycobiology 1. Canadian Biological Publication, Guelph, p 140
Chartier C, Paraud C (2004) Biological control of nematodes in goats with Duddingtonia flagrans, from laboratory to field trials: promising results yet to be confirmed. International workshop “sustainable, non-chemical control of small ruminant nematode parasites in Europe”, Frederiksberg, Denmark, 20–21 October, pp 17–20
Chartier C, Pors I (2003) Effect of the nematophagous fungus, Duddingtonia flagrans, on the larval development of goat parasitic nematodes: a plot study. Vet Res 34:221–230
Chartier C, Reche B (1992) Gastrointestinal helminths and lungworms of French dairy goats: prevalence and geographical distribution in Poitou-Charentes. Vet Res Commun 16:327–335
Chartier C, Soubirac F, Pors I, Silvestre A, Hubert J, Couquet C, Cabaret J (2001) Prevalence of anthelmintic resistance in gastrointestinal nematodes of dairy goats under extensive management conditions in southwestern France. J Helminthol 75:325–330
Cheah TS, Rajamanickam C (1997) Epidemiology of gastro-intestinal nematodes of sheep in wet tropical conditions in Malaysia. Trop Anim Health Prod 29:165–173
Ciordia H, Bizzell WE (1963) The effects of various constant temperatures on the development of the free-living stages of some nematode parasites of cattle. J Parasitol 49:60–63
Cooke RC (1968) Relationships between nematode-destroying fungi and soil-borne phytonematodes. Phytopathology 58:904–913
Doumenc V, Pors I, Chartier C (2004) Helminth fauna of dairy goats in Burgundy area (France): relationships with mixed or alternate grazing with beef cattle. 8th International Conference on Goats, Pretoria, Afrique du Sud, 4–9 July, N.058, p 124
Duddington CL (1957) The predacious fungi and their place in microbial ecology. Symp Soc Gen Microbiol 218–237
Fernández AS, Larsen M, Wolstrup J, Grønvold J, Nansen P, Bjørn H (1999) Growth rate and trapping efficacy of nematode-trapping fungi under constant and fluctuating temperatures. Parasitol Res 85:661–668
Grønvold J, Nansen P, Henriksen SA, Larsen M, Wolstrup J, Bresciani J, Rawat H, Fribert L (1996) Induction of traps by Ostertagia ostertagi larvae, chlamydospore production and growth rate in the nematode-trapping fungus Duddingtonia flagrans. J Helminthol 70:291–297
Gruner L, Berbigier P, Cortet J, Sauve C (1989) Effects of irrigation on appearance and survival of infective larvae of goat gastro-intestinal nematodes in Guadeloupe (French West Indies). Int J Parasitol 19:409–415
Hoste H, Cabaret J (1992) Intergeneric relations between nematodes of the digestive tract in lambs: a multivariate approach. Int J Parasitol 22:173–179
Hoste H, Chartier C (1993) Effect on milk production of concurrent infection with Haemonchus contortus and Trichostrongylus colubriformis in dairy goats: comparison in animals with high and low levels of production. Am J Vet Res 54: 1886–1893
Hoste H, Paraud C, Paolini V, Chartier C (2004) Gestion non médicale du parasitisme par les nématodes digestifs: méthodes alternatives à la chimiothérapie. Journées Nationales des Groupements Techniques Vétérinaires, Tours, France, 26–28 mai 2004, 41–45
Kaplan RM (2004) Drug resistance in nematodes of veterinary importance: a status report. Trends Parasitol 20: 477–481
Larsen M (2000) Prospects for controlling animal parasitic nematodes by predacious micro fungi. Parasitology 120 Suppl:S121–131
Morgan M, Behnke JM, Lucas JA, Peberdy JF (1997) In vitro assessment of the influence of nutrition, temperature and larval density on trapping of the infective larvae of Heligmosomoides polygyrus by Arthrobotrys oligospora, Duddingtonia flagrans and Monacrosporium megalosporum. Parasitology 115:303–310
Nansen P, Grønvold J, Henriksen SA, Wolstrup J (1988) Interactions between the predacious fungus Arthrobotrys oligospora and third-stage larvae of a series of animal-parasitic nematodes. Vet Parasitol 26:329–337
Pandey VS, Chaer A, Dakkak A (1993) Effect of temperature and relative humidity on survival of eggs and infective larvae of Ostertagia circumcincta. Vet Parasitol 49:219–227
Paraud C, Chartier C (2003) Biological control of infective larvae of a gastro-intestinal nematode (Teladorsagia circumcincta) and a small lungworm (Muellerius capillaris) by Duddingtonia flagrans in goat faeces. Parasitol Res 89:102–106
Paraud C, Pors I, Chartier C (2004) Activity of Duddingtonia flagrans on Trichostrongylus colubriformis larvae in goat faeces and interaction with a benzimidazole treatment. Small Rumin Res 55: 199–207
Paraud C, Hoste H, Lefrileux Y, Pommaret A, Paolini V, Pors I, Chartier C (2005) Administration of Duddingtonia flagrans chlamydospores to goats to control gastro-intestinal nematodes: dose trials. Vet Res 36:157–166
Raynaud JP (1970) Etude de l’efficacité d’une technique de coproscopie quantitative pour le diagnostic de routine et le contrôle des infestations parasitaires des bovins, ovins, équins et porcins. Ann Parasitol Hum Comp 45:321–342
Rossanigo CE, Gruner L (1995) Moisture and temperature requirements in faeces for the development of free-living stages of gastrointestinal nematodes of sheep, cattle and deer. J Helminthol 69:357–362
Terrill TH, Larsen M, Samples O, Husted S, Miller JE, Kaplan RM, Gelaye S (2004) Capability of the nematode-trapping fungus Duddingtonia flagrans to reduce infective larvae of gastro-intestinal nematodes in goat feces in the southeastern United States: dose titration and dose time interval studies. Vet Parasitol 120:285–296
Thamsborg SM, Roepstorff A, Larsen M (1999) Integrated and biological control of parasites in organic and conventional production systems. Vet Parasitol 84: 169–186
Waghorn TS, Leathwick DM, Chen LY, Skipp RA (2003) Efficacy of the nematode-trapping fungus Duddingtonia flagrans against three species of gastro-intestinal nematodes in laboratory faecal cultures from sheep and goats. Vet Parasitol 118:227–234
Waller P (1999) International approaches to the concept of integrated control of nematode parasites of livestock. Int J Parasitol 29:155–164
Waller P, Faedo M (1993) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: screening studies. Vet Parasitol 49:285–297
Wright DA, McAnulty RW, Noonan MJ, Stankiewicz M (2003) The effect of Duddingtonia flagrans on trichostrongyle infections of Saanen goats on pasture. Vet Parasitol 118:61–69
Acknowledgements
This experiment was supported by the European project FAIR QLK5-CT-2001-01843, “Worm control in organic production systems for small ruminants in Europe: towards the implementation of non-chemical, sustainable approaches”, which is a collaboration between Denmark, Sweden, Spain, UK, The Netherlands and France. C. Paraud is a grateful recipient of a grant from AFSSA/Région Poitou-Charentes. We also thank Christian Hansen Ltd. for providing Duddingtonia flagrans chlamydospores.
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Paraud, C., Pors, I., Chicard, C. et al. Comparative efficacy of the nematode-trapping fungus Duddingtonia flagrans against Haemonchus contortus, Teladorsagia circumcincta and Trichostrongylus colubriformis in goat faeces: influence of the duration and of the temperature of coproculture. Parasitol Res 98, 207–213 (2006). https://doi.org/10.1007/s00436-005-0028-4
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DOI: https://doi.org/10.1007/s00436-005-0028-4