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Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers

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Abstract

The phylogenetic relationships among 31 different flea isolates representing seven different species were studied by nucleotide sequence comparison of the internal transcribed spacer 1 (ITS1), internal transcribed spacer 2 (ITS2) and/or mitochondrial 16S ribosomal RNA gene (mt16S-rDNA) to examine the patterns of variation. Results show that all regions are useful in discriminating among flea species. In Ctenocephalides felis and Tunga penetrans, some differences in these gene regions occurred among different isolates within the same species. In the latter case, the differences are in the mt16S-rDNA region, with one isolate showing 48% divergence in nucleotide sequence. The taxonomic implications of this result are unclear at present. The gene regions revealed differences between C. felis isolates only after DNA sequencing the PCR products. Further differentiation among C. felis isolates was obtained using four different random binding primers (decamers) and primers for mammalian aldolase to amplify narrow differences in the genome. Using these primers we were able to discriminate between different C. felis isolates and determine that some of the genetic variation coincided with minor differences in response to the control agent imidacloprid. However, overall findings do not support the existence of subspecies of C. felis.

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References

  • Baldwin B, Sanderson MJ, Porter JM, Wojciechowski MF, Campbell CS, Donoghue MJ (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Mo Bot Gard 82:247–277

    Google Scholar 

  • Coleman AW, Mai JC (1997) Ribosomal DNA ITS-1 and ITS-2 sequence comparisons as a tool for predicting genetic relatedness. J Mol Evol 45:68–177

    Google Scholar 

  • Essig A, Rinder H, Gothe R, Zahler M (1999) Genetic differentiation of mites of the genus Chorioptes (Acari: Psoroptidae). Exp Appl Acarol 23:309–318

    Article  CAS  PubMed  Google Scholar 

  • Haeselbarth E (1966) A note on the subspecies of C. felis in Africa south of the Sahara. Zool Anz 176:357–365

    Google Scholar 

  • Haymer DS, McInnis DO (1994) Resolution of populations of the Mediterranean fruit fly at the DNA level using random primers for the polymerase chain reaction. Genome 37:244–248

    CAS  PubMed  Google Scholar 

  • Heise M, Epe C, Schnieder T (1999) Differences in the second internal transcribed spacer (ITS-2) of eight species of gastrointestinal nematodes of ruminants. J Parasitol 85:431–435

    Article  CAS  PubMed  Google Scholar 

  • Hopkins GHE, Rothschild M (1953) An illustrated catalogue of the Rothschild collection of fleas in the British Museum, Vol 1. University Press, Cambridge

  • Krämer F, Mencke N (2001) Flea biology and control. Springer, Berlin Heidelberg New York

  • Lane RP, Crosskey RW (eds) (1993) Medical insects and arachnids. Chapman and Hall, London

  • Marcilla A, Bargues MD, Abad-Franch F, Panzera F, Carcavallo RU, Noireau F, Galvao C, Jurberg J, Miles MA, Dujardin JP, Mas-Coma S (2002) Nuclear rDNA ITS-2 sequences reveal polyphyly of Panstrongylus species (Hemiptera: Reduviidae: Triatominae), vectors of Trypanosoma cruzi. Infect Genet Evol 1:225–235

    Article  CAS  PubMed  Google Scholar 

  • McIntosh AH, Grasela JJ, Matteri RL (1996) Identification of insect cell lines by DNA amplification fingerprinting (DAF). Insect Mol Biol 5:187–195

    CAS  PubMed  Google Scholar 

  • Mehlhorn H (ed) (2001) Encyclopedic references of parasitology. Springer, Berlin Heidelberg New York

  • Motazedian H, Noyes H, Maingon R (1996) Leishmania and Sauroleishmania: the use of random amplified polymorphic DNA for the identification of parasites from vertebrates and invertebrates. Exp Parasitol 83:150–154

    Article  CAS  PubMed  Google Scholar 

  • Oliveros R, Cutillas C, De Rojas M, Arias P (2000) Characterization of four species of Trichuris (Nematoda: Enoplida) by their second internal transcribed spacer ribosomal DNA sequence. Parasitol Res 86:1008–1013

    CAS  PubMed  Google Scholar 

  • Parker A, Kornfield I (1996) An improved amplification and sequencing strategy for phylogenetic studies using the mitochondrial large subunit rRNA gene. Genome 39:793–797

    CAS  PubMed  Google Scholar 

  • Rinder H, Rausch RL, Takahashi K, Kopp H, Thomschke A, Löscher T (1997) Limited range of genetic variation in Echinococcus multilocularis. J Parasitol 83:1045–1050

    CAS  PubMed  Google Scholar 

  • Rust MK, Waggoner M, Hinkle NC, Mencke N, Hansen O, Vaughn M, Dryden MW, Payne P, Blagburn B, Jacobs DE, Bach T, Bledsoe D, Hopkins T, Mehlhorn H (2002) Development of a larval bioassay for the susceptibility of cat fleas (Siphonaptera: Pulicidae) to imidacloprid. J Med Entomol 39:671–674

    CAS  PubMed  Google Scholar 

  • Shouche YS, Patole MS (2000) Sequence analysis of mitochondrial 16S ribosomal RNA gene fragment from seven mosquito species. J Biosci 25:361–366

    CAS  PubMed  Google Scholar 

  • Vobis M, D’Haese J, Mehlhorn H, Feldmeier H, Heukelbach, Mencke N (2004) Molecular biological investigation of Brazilian Tunga penetrans isolates from man, dogs and cats. Parasitol Res (in press)

  • Whitfield JB, Cameron SA (1998) Hierarchical analysis of variation in the mitochondrial 16S rRNA gene among Hymenoptera. Mol Biol Evol 15:1728–1743

    CAS  PubMed  Google Scholar 

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Correspondence to H. Mehlhorn.

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Vobis, M., D’Haese, J., Mehlhorn, H. et al. Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers. Parasitol Res 94, 219–226 (2004). https://doi.org/10.1007/s00436-004-1201-x

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  • DOI: https://doi.org/10.1007/s00436-004-1201-x

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