Duffy antigen is important for the lethal effect of the lethal strain of Plasmodium yoelii 17XL
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We studied the potential role of the Duffy antigen and glycophorin A as receptors for rodent malaria parasite invasion of erythrocytes. Parasitemia increased exponentially after infection with Plasmodium berghei NK65, P. chabaudi, and P. vinckei in Duffy antigen knockout, glycophorin A knockout, and wild-type mice, indicating that the Duffy antigen and glycophorin A are not essential for these malaria parasites. However, parasitemia of the Duffy antigen knockout mice infected with P. yoelii 17XL remained constant from day 5 to 14 after infection, and then decreased, resulting in autotherapy. The treatment of P. yoelii 17XL-infected Duffy antigen knockout mice with anti-CD4 antibody increased the parasitemia 15 days after infection and the mice eventually died, indicating that CD-4-positive cells play an important role in the clearance of P. yoelii 17XL at the late stage of the infection.
KeywordsMalaria Parasite Erythrocyte Membrane Protein Tail Blood Rodent Malaria Parasite Duffy Antigen
We thank Dr. T. Yoshimoto and Dr. S. Kaneko for helpful discussions. We thank Dr. S. Waki for providing the malaria parasites P. vinckei and P. chabaudi. We also thank Dr. I. Igarashi for providing the anti-CD4 and anti-CD8 antibodies. This work was partially supported by a Grant-in-Aid for Scientific Research on priority Areas (12307007 and 14021072) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The experiments comply with the current laws of the countries in which the experiments were performed.