Skip to main content
Log in

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

When in vitro cultivation was used as the ‘gold standard’ for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Ashford RW, Atkinson EA (1992) Epidemiology of Blastocystis hominis infection in Papua New Guinea: age-prevalence and associations with other parasites. Ann Trop Med Parasitol 86:129–136

    CAS  PubMed  Google Scholar 

  • Böhm-Gloning B, Knobloch J, Walderich B (1997) Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop Med Int Health 2:771–778

    Article  PubMed  Google Scholar 

  • Clark CG (1997) Extensive genetic diversity in Blastocystis hominis. Mol Biochem Parasitol 87:79–83

    Article  CAS  PubMed  Google Scholar 

  • Garcia SL, Bruckner DA (1997) Macroscopic and microscopic examination of fecal specimens. In: Garcia SL, Bruckner DA (eds) Diagnostic medical parasitology, 3rd edn. American Society for Microbiology, Washington, DC., pp 608–651,

  • Hussain Qadri SM, Al-Okaili GA, Al-Dayel F (1989) Clinical significance of Blastocystis hominis. J Clin Microbiol 27:2407–2409

    PubMed  Google Scholar 

  • Jones WR (1946) The experimental infection of rats with Entamoeba histolytica. Ann Trop Med Parasitol 40:130–140

    Google Scholar 

  • Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M (2002) In vitro cultivation: a sensitive method for detecting Blastocystis hominis. Ann Trop Med Parasitol 96:803–807

    Article  CAS  PubMed  Google Scholar 

  • Nimri LF (1993) Evidence of an epidemic of Blastocystis hominis infections in preschool children in northern Jordan. J Clin Microbiol 31:2706–2708

    CAS  PubMed  Google Scholar 

  • Noël C, Peyronnet C, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Sogin ML, Capron M, Viscogliosi E, Zenner L (2003) Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Mol Biochem Parasitol 126:119–123

    Article  PubMed  Google Scholar 

  • Stenzel DJ, Boreham PFL (1996) Blastocystis hominis revisited. Clin Microbiol Rev 9:563–584

    CAS  PubMed  Google Scholar 

  • Taamasri P, Leelayoova S, Rangsin R, Naaglor T, Ketupanya A, Mungthin M (2002) Prevalence of Blastocystis hominis carriage in Thai army personnel based in Chonburi, Thailand. Mil Med 167:643–646

    PubMed  Google Scholar 

  • Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S (2003) Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. J Clin Microbiol 41:967–975

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the Thailand Tropical Diseases Research Programme (T2) (ID 00-1-HEL-24-011). The experiments comply with the current laws of Thailand.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mathirut Mungthin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Termmathurapoj, S., Leelayoova, S., Aimpun, P. et al. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol Res 93, 445–447 (2004). https://doi.org/10.1007/s00436-004-1157-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-004-1157-x

Keywords

Navigation