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Prevalence of Sarcocystis species sporocysts in Northern Virginia opossums (Didelphis virginiana)

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Abstract

A total of 206 Virginia opossums (Didelphis virginiana) collected from the mid-Michigan region, United States, during a period extending from 1996 to 2002 were sampled for the presence of Sarcocystis spp sporocysts. All isolates were phenotypically identified as Sarcocystis spp and genotyped to the species level by PCR-based techniques. The overall prevalence of Sarcocystis spp in opossums was 18% (37/206). The prevalence of Sarcocystis spp differed significantly with age (P<0.001) and adult opossums were more commonly infected (14.6%; 30/206) than juveniles (3.4%; 7/206). No significant difference in the prevalence of Sarcocystis spp infection was observed between male and female (P<0.15). The highest prevalence was recorded during summer (9.2%; 19/206). PCR-RFLP analyses demonstrated the majority of Sarcocystis isolates to be S. neurona, with some animals co-infected with sporocysts of S. falcatula. Out of the 37 Sarcocystis-infected opossums, 23 (62%) had sporocysts of S. neurona only, four (11%) had sporocysts of S. falcatula only, and eight (22%) had a mixture of S. neurona and S. falcatula sporocysts. These findings indicate that mixed Sarcocystis infections in opossums are common. The propensity for Sarcocystis spp to co-exist in the opossum gut enhances dissemination and environmental contamination with these coccidia. Additionally, this increases the chance for sexual recombination between Sarcocystis spp, given the proclivity of these species to reproduce sexually at high numbers in the intestinal cells of their definitive host.

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Acknowledgements

This work was supported by a grant from the Grayson Jockey-Club Research Foundation. The study was carried out in compliance with the law and regulations of the United States.

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Authors and Affiliations

  1. Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA

    Hany M. Elsheikha & Linda S. Mansfield

  2. Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI 48824, USA

    Alice J. Murphy

  3. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA

    Linda S. Mansfield

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  1. Hany M. Elsheikha
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  2. Alice J. Murphy
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  3. Linda S. Mansfield
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Correspondence to Hany M. Elsheikha.

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Elsheikha, H.M., Murphy, A.J. & Mansfield, L.S. Prevalence of Sarcocystis species sporocysts in Northern Virginia opossums (Didelphis virginiana). Parasitol Res 93, 427–431 (2004). https://doi.org/10.1007/s00436-004-1150-4

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  • DOI: https://doi.org/10.1007/s00436-004-1150-4

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