Abstract
Cryptosporidium is a significant pathogen in humans and animals. Cases of infection by C. meleagridis or C. baileyi with zoonotic potential have also been reported in domestic birds; and recent studies indicate the presence of new host-adapted species or genotype in birds. Therefore, accurately identifying isolates is important for understanding the epizootiology of Cryptosporidium infection in birds and for the control of human cryptosporidiosis. Cryptosporidium has been detected in cockatiels, but the species or genotype of isolates remains unclear because identification was performed using conventional microscopy. We report herein the species or genotype of isolates from two cockatiels distinguished by a PCR-based diagnostic method. The isolates were found to be C. meleagridis and C. baileyi, respectively. This study documents the first discovery of C. meleagridis and C. baileyi in cockatiels and suggests that pet birds may play an important role in the epidemiology of cryptosporidiosis.
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References
Abe N, Kimata I, Iseki M (2002a) Identification of genotypes of Cryptosporidium parvum isolates from a patient and a dog in Japan. J Vet Med Sci 64:165–168
Abe N, Sawano Y, Yamada K, Kimata I, Iseki M (2002b) Cryptosporidium infection in dogs in Osaka, Japan. Vet Parasitol 108:187–195
Abe N, Kimata I, Iseki M (2002c) Comparative study of PCR-based Cryptosporidium discriminating techniques with a review of the literature. Kansenshogaku Zasshi 76:869–881
Akiyoshi DE, Dilo J, Pearson C, Chapman S, Tumwine J, Tzipori S (2003) Characterization of Cryptosporidium meleagridis of human origin passaged through different host species. Infect Immun 71:1828–1832
Cai J, Collins MD, McDonald V, Thompson DE (1992) PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasites Cryptosporidium parvum and Cryptosporidium muris. Biochim Biophys Acta 1131:317–320
Ditrich O, Palkovic L, Sterba J, Prokopic J, Loudová J, Giboda M (1991) The first finding of Cryptosporidium baileyi in man. Parasitol Res 77:44–47
Goodwin MA, Krabill VA (1989) Diarrhea associated with small-intestinal cryptosporidiosis in a budgerigar and in a cockatiel. Avian Dis 33:829–833
Johnson DW, Pieniazek NJ, Griffin DW, Misener L, Rose JB (1995) Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol 61:3849–3855
Kimbell LM, Miller DL, Chavez W, Altman N (1999) Molecular analysis of the 18S rRNA gene of Cryptosporidium serpentis in a wild-caught corn snake (Elaphe guttata guttata) and a five-species restriction fragment length polymorphism-based assay that can additionally discern C. parvum from C. wrairi. Appl Environ Microbiol 65:5345–5349
Lindsay DS, Blagburn BL, Hoerr FJ (1990) Small intestinal cryptosporidiosis in cockatiels associated with Cryptosporidium baileyi-like oocysts. Avian Dis 34:791–793
Morgan UM, Xiao L, Fayer R, Graczyk TK, Lal AA, Deplazes P, Thompson RCA (1999) Phylogenetic analysis of Cryptosporidium isolates from captive reptiles using 18S rDNA sequence data and random amplified polymorphic DNA analysis. J Parasitol 85:525–530
Morgan UM, Xiao L, Limor J, Gelis S, Raidal SR, Fayer R, Lal A, Elliot A, Thompson RCA (2000) Cryptosporidium meleagridis in an Indian ring-necked parrot (Psittacula krameri). Aust Vet J 78:182–183
Morgan UM, Monis PT, Xiao L, Limor J, Sulaiman I, Raidal S, O’Donoghue P, Gasser R, Murray A, Fayer R, Blagburn BL, Lal AA, Thompson RCA (2001) Molecular and phylogenetic characterization of Cryptosporidium from birds. Int J Parasitol 31:289–296
O’Donoghue PJ (1995) Cryptosporidium and cryptosporidiosis in man and animals. Int J Parasitol 25:139–195
Perriere G, Gouy M (1996) WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369
Saitou N, Nei M (1987) The neighbor joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Satou M, Hikosaka K, Sasaki T, Suyama Y, Yanai T, Ohta M, Nakai Y (2003) Characterization of a novel type of bovine Cryptosporidium andersoni. Appl Environ Microbiol 69:691–692
Sréter T, Varga I (2000) Cryptosporidiosis in birds. Vet Parasitol 87:261–279
Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RCA, Fayer R, Lal AA (1999) Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 65:3386–3391
Xiao L, Morgan UM, Fayer R, Thompson RCA, Lal AA (2000) Cryptosporidium systematics and implications for public health. Parasitol Today 16:287–292
Xiao L, Sulaiman IM, Ryan UM, Zhou L, Atwill ER, Tischler ML, Zhang X, Fayer R, Lal AA (2002) Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Int J Parasitol 32:1773–1785
Yagita K, Izumiyama S, Tachibana H, Masuda G, Iseki M, Furuya K, Kameoka Y, Kuroki T, Itagaki T, Endo T (2001) Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan. Parasitol Res 87:950–955
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Abe, N., Iseki, M. Identification of Cryptosporidium isolates from cockatiels by direct sequencing of the PCR-amplified small subunit ribosomal RNA gene. Parasitol Res 92, 523–526 (2004). https://doi.org/10.1007/s00436-004-1082-z
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DOI: https://doi.org/10.1007/s00436-004-1082-z