Abstract
Scanning electron microscopy was used to study the surface ultrastructure of adult worms of Spirocerca lupi. At the anterior end of both sexes, the mouth is hexagonal, without definite lips. Around the mouth, two pairs of submedian cephalic papillae and two lateral amphids are present. There is a pair of lateral cervical papillae. At the anterior end of the body, on the ventral side, an excretory pore is seen. In the female, the vulva is located anteriorly and the tail is blunt, with a pair of subterminal phasmids. The male tail is spiral, with four pairs of preanal papillae, a single large median preanal papilla on the anterior lip of the cloacal aperture, two pairs of postanal papillae, four pairs of tiny papillae near the tip of the tail and two subterminal phasmids. The cuticle shows an abnormal tumour-like mass in two cases.
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References
Anderson RC (2000) Nematode parasites of vertebrates, their development and transmission. CABI, UK
Ashour AA, Lewis JW (1986) Gongylonema aegypti n. sp. (Nematoda: Thelaziidae) from Egyptian rodents. Syst Parasitol 8:199–206
Bailey WS (1972) Spirocerca lupi: a continuing inquiry. J Parasitol 58:3–22
Beelitz P, Dongus H, Schol H, Gerhards H (1997) Thelazia lacrymalis (Nematoda, Spirurida, Thelaziidae): report in a horse in Germany and contribution to the morphology of adult worms. Parasitol Res 83:627–631
Berry WL (2000) Spirocerca lupi esophageal granulomas in 7 dogs: resolution after treatment with doramectin. J Vet Intern Med 14:609–612
Bowmann DD, Lynn RC (1999) Georgis’ parasitology for veterinarians, 7th edn. Saunders, Philadelphia, Pa., USA
Chabaud AG (1975) Keys to the genera of the order Spirurida. Part 2. Spiruroidea, Habronematoidea and Acuarioidea. In: Anderson RC, Chabaud AG, Willmott S (eds) CIH Keys to the nematode parasites of vertebrates, vol 3. Commonwealth Agricultural Bureaux, England
Chakraborty A (1994) Occurrence and pathology of Gongylonema infection in captive wild herbivores. Vet Parasitol 52:163–167
Choi WY, Youn JH, Nam HW, Kim SW, Kim WK, Park SY, Oh YW (1989) Scanning electron microscopic observations of Thelazia callipaeda from human. Kisaengch’unghak Chapchi. (Korean J Parasitol) 27:217–223
Chowdhury N, Pande BP (1969) The development of the infective larva of the canine oesophageal tumor worm Spirocerca lupi in rabbits and its histophathology. Z Parasitenkd 32:1–10
Giannetto S, Trotti GC (1995) Light and scanning electron microscopy of Spirura rytipleurites seurati Chabaud, 1954 (Nematoda: Spiruridae) from Erinaceus europaeus in Sicily. J Helminthol 69:305–311
Gibbons LM (1986) SEM Guide to the morphology of nematode parasites of vertebrates. CABI, UK
Guttekova A (1987) Ultrastructure of the surface sculpture of the nematodes Thelazia gulosa and Thelazia rhodesi. Vet Med 32:113–120
Hamir AN (1984) Perforation of thoracic aorta in a dog associated with Spirocerca lupi infection. Aust Vet J 61:64
Hamir AN ( 1986) Oesophageal perforation and pyothorax associated with Spirocerca lupi infestation in a dog. Vet Rec 119:276
Kutzer E, Burtscher H (1967) Rarely occurring endoparasites of domestic dogs and cats. I. Spirocerca lupi. Wien Tierarztl Monatsschr 54:542–546
Levine ND (1968) Nematode parasites of domestic animals and man. Burgess, USA
McLaren DJ (1974) The anterior glands of Necator americanus (Nematoda: Strongyloides). Ultrastructural studies. Int J Parasitol 4:25–34
McLaren DJ (1976) Nematode sense organs. Adv Parasitol 14:105–265
Murray M (1968) Incidence and pathology of Spirocerca lupi in Kenya. J Comp Pathol 78:401–405
Naem S, Seifi H, Simon GT (2000) Scanning electron microscopy of adult Gongylonema pulchrum (Nematoda: Spirurida). J Vet Med B 47:249–255
Oshmarin PG, Oshmarin AP, Ryzhikov KM, Khrustalev AV (1983) Structure and nutritional characteristics of the female Simondsia paradoxa (Nematoda, Spirurata) a gastric parasite of swine. Parazitologiia 17:448–458
Pence DB, Stone JE (1978) Visceral lesions in wild carnivores naturally infected with Spirocerca lupi. Vet Pathol 15:322–331
Schroeder H, Berry WL (1998) Salivary gland necrosis in dogs: a retrospective study of 19 cases. J Small Anim Pract 39:121–125
Skrjabin KI (1969) Key to parasitic nematodes, vol 1. Spirurata and Filariata. Academy of Sciences USSR, Moscow
Soulsby EJL (1965) Textbook of veterinary clinical parasitology, vol 1. Helminths. Blackwell, Oxford, UK
Soulsby EJL (1986) Helminths, arthropods and protozoa of domesticated animals. Baillier Tindall, London, UK
Stephens LC, Gleiser CA, Jardine JH (1983) Primary pulmonary fibrosarcoma associated with Spirocerca lupi infection in a dog with hypertrophic pulmonary osteoarthropathy. J Am Vet Med Assoc 182:496–498
Talbot NT (1971) Incidence of Spirocerca lupi in dogs in the Port Moresby area of Papua and New Guinea. Aust Vet J 47:189–191
Uni S, Abe M, Harada K, Kanedak K, Kimata I, Abdelmaksoud NH, Takahashi K, Miyashita H, Iseki H (1992) New record of Gongylonema pulchrum Molin, 1857 from a new host, Macaca fuscata, in Japan. Ann Parasitol Hum Comp 67:221–223
Wandera JG (1976) Further observations on canine spirocercosis in Kenya. Vet Rec 99:348–351
Yamaguti S (1961) Systema helminthum, vol 3. The nematodes of vertebrates, parts 1 and 2. Interscience, New York, USA
Acknowledgements
The author wish to express her appreciation to Professor Larry A. Arsenault, Head of the Electron Microscope Facility, Department of Pathology and Molecular Medicine, McMaster University, Canada for his assistance in providing all facilities during the study. Also, the author is grateful to Mr. Ernie Spitzer, Chief Technician, and to all technicians in the Electron Microscope Facility for their help. Meanwhile, thanks are due to Dr. Hamed Seifi and Dr. H. A. Seifi for critical review of the manuscript.
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Naem, S. Scanning electron microscopic observations on adult Spirocerca lupi (Nematoda: Spirurida, Thelaziidae). Parasitol Res 92, 265–269 (2004). https://doi.org/10.1007/s00436-003-1037-9
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DOI: https://doi.org/10.1007/s00436-003-1037-9