Abstract
Gnathostoma spinigerum infection is endemic in Thailand and many Asian countries. Current diagnosis is the skin test and enzyme-linked immunosorbent assay (ELISA) for IgG antibody against the G. spinigerum third-stage larvae (L3), but cross-reactivity is common. We evaluated the sensitivity and specificity of anti-G. spinigerum L3 IgG subclass antibodies for diagnosis of 43 patients with gnathostomiasis. The majority of patients with gnathostomiasis (91%) had eosinophilia. While the anti-G. spinigerum L3 IgG1 antibody provided the highest sensitivity (98%), the anti-G. spinigerum L3 IgG2 antibody had the highest specificity (88%). The ELISA that detected anti-G. spinigerum L3 IgG1 antibody could be a reliable laboratory screening test, while anti-G. spinigerum L3 IgG2 antibody could be used to confirm the diagnosis.
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Acknowledgements
This study was supported by Ratchadapisek-sompotch Fund, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. We would like to thank Ms. Narak Triteeraprapab for data analysis. We are thankful to all staff at the Hematology unit, the Department of Pediatrics, and the Department of Parasitology, Faculty of Medicine, Chulalongkorn University, for their technical help.
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Nuchprayoon, S., Sanprasert, V., Suntravat, M. et al. Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum . Parasitol Res 91, 137–143 (2003). https://doi.org/10.1007/s00436-003-0947-x
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DOI: https://doi.org/10.1007/s00436-003-0947-x