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Immunoblot analysis of Trichomonas vaginalis antigens recognized by rabbit hyperimmune serum raised against exoantignes

Abstract

The SDS-PAGE and immunoblot were used to identify Trichomonas vaginalis antigenic epitopes present in purified somatic and exo-antigens. The presence of common immunogenic proteins corresponding to molecular weights of 76, 60 and 23 kDa was revealed. Distinctive immunogenic bands of 92, 72, 55 and 40 kDa for the exo-antigens, and of 110, 80, 78 and 50 kDa for the somatic antigens, appeared when the antigens were probed by the homologous immune rabbit serum.

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Fig. 1.

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Acknowledgements

We are grateful to the Parasitology Diagnostic Unit and Medical Research Center, Faculty of Medicine, Ain Shams University, Cairo, Egypt, for giving us free use of all the facilities of their laboratories while carrying out this study. We herewith declare that the experiments comply with the current laws and regulations of the Egyptian Ministry of Higher Education and Scientific Research.

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Correspondence to Magda E. Azab.

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Khalifa, K.E., El-Wakil, H.S.I., Habib, F.S. et al. Immunoblot analysis of Trichomonas vaginalis antigens recognized by rabbit hyperimmune serum raised against exoantignes. Parasitol Res 92, 48–49 (2004). https://doi.org/10.1007/s00436-003-0940-4

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Keywords

  • Antigenic Epitope
  • Vaginal Discharge
  • Trichomoniasis
  • Preliminary Attempt
  • Immunogenic Protein