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Species-specific proteins identified in Ascaris lumbricoides and Ascaris suum using two-dimensional electrophoresis

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Abstract.

The protein profile of adult female Ascaris lumbricoides and Ascaris suum originating from humans and pigs, respectively, was studied using two-dimensional polyacrylamide gel electrophoresis. Six different major protein spots specific for A. lumbricoides were identified irrespective of their geographical origin and no major specific spot was encountered in A. suum. No major differences in the protein profiles between the extract by phosphate-buffered saline and urea were encountered for either Ascaris species. It is therefore possible to use 2D-PAGE as a tool for discriminating the closely related Ascaris species from humans and pigs.

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Authors and Affiliations

  1. Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricultural Research Organization, 3–1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan, , , , ,

    Wossene Abebe, Naotoshi Tsuji, Harue Kasuga-Aoki, Takeharu Miyoshi, Takashi Isobe & Shinobu Yoshihara

  2. Department of Parasitology, School of Medicine, University of Ryukyus, Uehara, Nishihara, Tyuto, Okinawa 903-0215, Japan, , , , ,

    Takeshi Arakawa

  3. Laboratory of Global Animal Resource Science, Graduate School of Agricultural Life Science, University of Tokyo, Yayoi, Bunkyo, Tokyo113–8657, Japan, , , , ,

    Yasunobu Matsumoto

Authors
  1. Wossene Abebe
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  2. Naotoshi Tsuji
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  3. Harue Kasuga-Aoki
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  4. Takeharu Miyoshi
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  5. Takashi Isobe
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  6. Takeshi Arakawa
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  7. Yasunobu Matsumoto
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  8. Shinobu Yoshihara
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Abebe, W., Tsuji, N., Kasuga-Aoki, H. et al. Species-specific proteins identified in Ascaris lumbricoides and Ascaris suum using two-dimensional electrophoresis. Parasitol Res 88, 868–871 (2002). https://doi.org/10.1007/s00436-002-0640-5

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  • DOI: https://doi.org/10.1007/s00436-002-0640-5

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