Effects of mercury and chromium upon longevity of Diplostomum sp. (Trematoda: Diplostomidae) cercariae

Abstract.

Laboratory experiments were carried out to examine the impact of mercury and chromium upon survival of Diplostomum sp. cercariae. Freshly emerged cercariae were exposed to solutions of either mercury, at concentrations ranging between 0.1 and 100 µg/l, or chromium at concentrations from 2 µg/l to 2 mg/l. The mortality patterns and mean survival times (MSTs) of the exposed parasites were determined by the Kaplan–Meier estimator and were compared (log-rank test) with corresponding patterns and estimators of the unexposed controls. Mercury concentrations ≥10 µg/l caused statistically significant changes in the mortality pattern of Diplostomum sp. cercariae and reduced MST by at least 9 h compared with the control group, in which MST was 42 h. Cercariae exposed to chromium solutions had statistically significant changes in mortality pattern only at a concentration of 2 mg/l and exhibited a reduction in MST of 8 h compared with the control (MST = 41 h). The results show for the first time that longevity of Diplostomum sp. cercariae can be reduced by direct exposure to heavy metals. Ecotoxicological effects of mercury are manifested at lower concentrations than those of chromium, and thus potentially have a higher impact on parasite populations and communities.