Renewal mechanisms of buccal armature in Flabellina verrucosa (Nudibranchia: Aeolidida: Flabellinidae)

Abstract

The general and fine morphology of the buccal armature and concomitant epithelia in Flabellina verrucosa were examined using light microscopy, cLSM, TEM, and SEM. A 3D-reconstruction of the radular sheath terminal end was constructed as well. Based on the obtained data, we suggest the mechanisms of jaw and radula syntheses for this species. The jaw plate’s growth is provided by the apocrine secretion of the gnathoblasts. There are two primary areas of jaw synthesis: the anterior area is responsible for growth formation of the jaw portion used in the masticatory process, and the posterior area provides the growth of the jaw plate. These types of synthesis (common for both areas) were described in detail for the first time for Gastropoda. The radula growth is provided by the microvillar activity of odontoblasts and membranoblasts. The rachidian tooth is synthesised by a single cell, and the lateral teeth are synthesised by group of 3–5 cells. The tooth formation includes four main stages: (1) tooth mould formation; (2) 90° turn of the tooth mould; (3) chitin accumulation, and (4) tooth maturation. The wide range of synthesis mechanisms is provided by a combination of two factors: the type of secretion and the amount of cells.

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Acknowledgements

The authors thank the divers’ team of the N. A. Pertsov White Sea Biological Station and also I. A. Ekimova (Department of Invertebrate Zoology, Biological faculty, Moscow State University) for providing material. Additionally, we are grateful to Dr. I. A. Kosevich and Dr. N. M. Biserova (Moscow State University) for help with different methods of microscopy. We want to thank the two unknown reviewers for most helpful comments on the typescript. The electron microscopy investigations were performed at the User Facilities Centre of Lomonosov Moscow State University and at the Institute for Biology of Inland Waters of the Russian Academy of Sciences. The present study was supported by Grants 16-04-00343 and 15-29-02447 from the Russian Foundation of Basic Research. The sampling trips and transmission electron microscopy investigations were supported by Russian Foundation for Basic Research Grant 15-04-02580 and by Grant of the President MK-6178.2015.4. The scanning electron microscopy investigations were supported by Russian Scientific Foundation Grant 14-50-00029.

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Correspondence to Anna Mikhlina.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Mikhlina, A., Tzetlin, A. & Vortsepneva, E. Renewal mechanisms of buccal armature in Flabellina verrucosa (Nudibranchia: Aeolidida: Flabellinidae). Zoomorphology 137, 31–50 (2018). https://doi.org/10.1007/s00435-017-0370-y

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Keywords

  • Cladobranchia
  • Ultrastructure
  • Radula
  • Jaws