Abstract
Effects of 3,3′,5′-triiodothyronine (rT3) in connection with 3,3′,5-triiodothyronine (T3) on 3T3 cells were studied in vitro by means of 1H and 31P NMR spectroscopy. In the cells incubated with 5 nM T3 for 3 h at pH 7.4, the ATP/ADP ratio was elevated from 6.9 to 8.4, whereas it was reduced to 6.1 in cells incubated with rT3. When the cells were incubated at pH 6.7, the ATP/ADP ratio was reduced to 6.6 and 5.2 at 1 and 2 h, respectively. In the presence of 5 nM of T3, however, the ratio was maintained above the control level. A 1-h preincubation with rT3 dramatically augmented the reductions caused by elevated acidity. These reductions were completely reversed when the cells were incubated with T3.
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Vitek V, Shatney CH, Lang DJ, Cowley RA (1983) Thyroid hormone responses in hemorrhagic shock: study in dogs and preliminary findings in humans. Surgery 93: 768–777
Vitek V, Shatney CH (1987) Thyroid hormone alterations in patients with shock and injury. Injury 18: 336–341
Sterling K, Lazarus JH, Milch PO, Sakurada T, Brenner MA (1978) Mitochondrial thyroid hormone receptor: localization and physiological significance. Science 201: 1126–1129
Oppenheimer JH (1979) Thyroid hormone: action at the cellular level. Science 203: 971–979
Green H, Kehinde O (1975) An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5: 19–27
Rozengurt E, Legg A, Strang G, Courtenay-Luck N (1981) Cyclic AMP: a mitogenic signal for Swiss 3T3 cells. Proc Natl Acad Sci USA 7: 4392–4396
Merchant TE, Gierke LW, Meneses P, Glonek T (1988) 31P magnetic resonance spectroscopic profiles of neoplastic human breast tissues. Cancer Res 48: 5112–5118
Pittman CS, Barker SB (1959) Inhibition of thyroxin action by 3,3′,5′-triiodothyronine. Endocrinology 64: 466–468
Coiro V, Harris A, Goodman HM, Vagenakis A, Braverman L (1990) Effect of pharmacological quantities of infused 3,3′,5′-triiodothyronine on thyroxine monodeiodination to 3,5,3′-triiodothyronine. Endocrinology 106: 69–75
Sterling K, Brenner MA, Sakurada T (1980) Rapid effect of triiodothyronine on the mitochondrial pathway in rat liver in vivo. Science 210: 340–343
Glynn IM, Karlish SJ (1975) The sodium pump. Annu Rev Physiol 37: 13–55
Segal J (1990) In vivo effect of 3,5,3′-triiodothyronine on calcium uptake in several tissues in the rat: evidence for a physiological role for calcium as the first messenger for the prompt action of thyroid hormone at the level of the plasma membrane. Endocrinology 127: 17–24
Markandeya J, Ewart HS, Brosnan JT (1992) Regulation of glycine catabolism in rat liver mitochondria. Biochem J 283: 435–439
O’Brien WE (1978) Inhibition of glycine synthase by branched-chain α-keto acids. Arch Biochem Biophys 189: 291–297
Hampson RK, Barron LL, Olson MS (1983) Regulation of the glycine cleavage system in isolated rat liver mitochondria. J Biol Chem 258:2993–2999
Horrum MA, Tobin RB, Ecklund RE (1991) Effects of thyroid hormones on the bypasses of the antimycin a block in the bc1 complex of rat liver mitochondria. Biochem Biophys Res Commun 178: 73–78
Novitzky D, Cooper DKC, Morrell D, Isaacs S (1988) Change from aerobic to anaerobic metabolism after brain death, and reversal following triiodothyronine therapy. Transplantation 45: 32–36
Ino K, Manaka D, Washida M, Yokoyama T, Okamoto R, Yamaoka Y, Ozawa K (1993) Effects of triiodothyronine on canine hepatic ischemia caused by Pringle’s maneuver. Surgery 113: 669–675
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Okamoto, R., Leibfritz, D. Adverse effects of reverse triiodothyronine on cellular metabolism as assessed by 1H and 31P NMR spectroscopy. Res. Exp. Med. 197, 211–217 (1997). https://doi.org/10.1007/s004330050070
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DOI: https://doi.org/10.1007/s004330050070