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Clinical significance of determining the hypermethylation of the RUNX3 gene promoter and its cohypermethylation with the BRCA1 gene for patients with breast cancer

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The aim of this study was to assess the clinical significance of RUNX3 gene hypermethylation in the pathogenetic mechanisms of breast cancer in women, taking into account its cohypermethylation with the BRCA1 gene.


This study included 74 women with newly diagnosed breast cancer (samples from female primary breast carcinomas and paired peripheral blood samples) and 62 women without oncological pathology—control group (peripheral blood samples). Epigenetic testing for hypermethylation status studying was performed in all samples on freshly collected material with the addition of a preservative before the storage and DNA isolation.


Hypermethylation of the RUNX3 gene promoter region was detected in 71.6% samples of breast cancer tissue and in 35.13% samples of blood. The RUNX3 gene promoter region hypermethylation was significantly higher among breast cancer patients compared to the control group. The frequency of cohypermethylation in RUNX3 and BRCA1 genes was significantly increased in breast cancer tissues compared to the blood of patients.


A significantly increased frequency of the hypermethylation of the RUNX3 gene promoter region and its cohypermethylation with the BRCA1 gene promoter region was found in tumor tissue and blood samples from patients with breast cancer, in contrast to the control group. The identified differences indicate the importance of further investigations of suppressor genes cohypermethylation in patients with breast cancer. Further large-scale studies are needed to find out whether the detected hypermethylation and cohypermethylation of the RUNX3 gene promoter region will have an impact on the treatment strategy in patients.

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All data generated or analyzed during this study are included in this published article.


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The authors declare that no funds, grants, or other support was received during the preparation of this manuscript.

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Authors and Affiliations



ZR, OL, and NG conceived this research and designed experiments; SP, CH, and RV participated in the design and interpretation of the data; VV, NM performed experiments and analysis; LF and ZR wrote the paper and participated in the revisions of it. All authors read and approved the final manuscript.

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Correspondence to Liliia Fishchuk.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Commission on Bioethical Expertise and Research Ethics of Bogomolets National Medical University (0120U100871).

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Informed consent for the collection of biological material, medical examinations, and data processing was obtained from each participant included in the study.

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Rossokha, Z., Fishchuk, L., Lobanova, O. et al. Clinical significance of determining the hypermethylation of the RUNX3 gene promoter and its cohypermethylation with the BRCA1 gene for patients with breast cancer. J Cancer Res Clin Oncol 149, 11919–11927 (2023).

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