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Impacts of potential anticancer agents based on pillar[5]arene for head and neck squamous cell carcinoma cells

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Abstract

Purpose

This study was conducted to investigate impacts of potential anticancer (associated with apoptosis and caspase pathways) of two newly synthesized derivatives of pillar[5]arene, named as d-Q-P5 and p-Q-P5, on Squamous cell carcinomas of the head and neck (HNSCC) cells.

Materials and methods

The MTT method was used to determine the IC50 doses of the derivatives on HNSCC cells, and the changes in gene expression were analyzed by real-time polymerase chain reaction (qPCR). The apoptosis change was confirmed by flow cytometry analysis.

Results

The results showed that the d-Q-P5 and p-Q-P5 effectively inhibited the proliferation of the cells by upregulating proapoptotic genes (Bax, Bad, p53, Bak, and Apaf-1) and genes involved in the caspase pathway (Casp2, Casp3, and Casp9), while downregulating the antiapoptotic gene (Bcl-2).

Conclusions

This study is the first to demonstrate the potential anticancer effects of these two agents on HNSCC cells by positively regulating apoptosis gene expression.

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Availability of data and materials

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

The study was supported by Necmettin Erbakan University (Project Number: 221218015).

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Authors and Affiliations

  1. Medicine Faculty, Department of Medical Biology, Karamanoglu Mehmetbey University, 70100, Karaman, Turkey

    Serkan Kuccukturk

  2. Meram School of Medicine, Department of Internal Medicine, Necmettin Erbakan University, 42080, Konya, Turkey

    Mehmet Ali Karaselek

  3. Medicine Faculty, Department of Medical Genetic, KTO Karatay University, 42020, Konya, Turkey

    Tugce Duran

  4. Science Faculty, Department of Chemistry, Selcuk University, 42250, Konya, Turkey

    Ahmed Nuri Kursunlu & Mustafa Ozmen

Authors
  1. Serkan Kuccukturk
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  2. Mehmet Ali Karaselek
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  3. Tugce Duran
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  4. Ahmed Nuri Kursunlu
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  5. Mustafa Ozmen
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Contributions

SK and TD contributed to anticancer activity experiments. MAK contributed to apoptosis experiments, methodology, writing—review & editing. ANK contributed to synthesis, methodology, writing—review & editing. MO contributed to characterization, methodology, writing—review & editing. The final version of the manuscript was read and approved by all the authors.

Corresponding author

Correspondence to Mustafa Ozmen.

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Conflict of interest

The authors declare that they have no conflicts of interest or competing interests.

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Not applicable.

Research involving human and animal participant

The study was performed on commercially available cell lines, not on living organisms.

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Kuccukturk, S., Karaselek, M.A., Duran, T. et al. Impacts of potential anticancer agents based on pillar[5]arene for head and neck squamous cell carcinoma cells. J Cancer Res Clin Oncol 149, 8711–8718 (2023). https://doi.org/10.1007/s00432-023-04822-y

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  • DOI: https://doi.org/10.1007/s00432-023-04822-y

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