Abstract
Purpose
Matrin 3 (MATR3) is a nuclear matrix protein involved in mRNA stabilization, nuclear retention of hyper-edited RNAs, and RNA splicing. The role of MATR3 in cancer is still unclear. The present study aimed to investigate expression levels and prognostic significance of MATR3 in stage I and II non-small cell lung cancer (NSCLC) patients.
Methods
We examined MATR3 protein immunohistochemically in tumoral and non-tumoral tissue sections from n = 67 NSCLC patients treated at hospital, and MATR3 mRNA from The Cancer Genome Atlas (TCGA) cohort with respect to valid prognostic and predictive features, as well as treatment outcome.
Results
Significantly higher immunohistochemical levels of MATR3 protein were found in tumor-adjacent tissue compared to cancer (p = 0.049). A decrease in MATR3 protein expression was found to be a significant independent adverse prognostic factor for patients overall survival (p = 0.007). By contrast, we observed higher MATR3 mRNA levels in tumoral tissue compared to control lung tissues (p < 0.001). Based on the TCGA dataset, we reported that high MATR3 mRNA level was significantly associated with worse OS of NSCLC patients (p < 0.001); however, it was not an independent prognostic marker (p = 0.156). The discrepancies in prognostic significance of MATR3 gene mRNA and protein levels imply a need for further investigation.
Conclusion
In conclusion, the present study warrants further investigation into the biological and prognostic value of MATR3 as a potential prognostic marker in early-stage NSCLC patients.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This research was funded by Nicolaus Copernicus University in Toruń, Faculty of Medicine, Collegium Medicum in Bydgoszcz; research task no. 141 within the framework of the basic research activity.
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Material preparation and data collection was performed by JD. Analyses were carried out by JD and AK-W. The staining evaluation was performed by JJ and DG. The formal analysis was conducted by PA and DG. The first draft of the manuscript was written by JD and KK. Supervision and review of the manuscript was conducted by MB. All authors read and approved the final manuscript.
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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Collegium Medicum in Bydgoszcz of Nicolaus Copernicus University in Torun (approval number KB 336/2018).
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Durślewicz, J., Klimaszewska-Wiśniewska, A., Jóźwicki, J. et al. Prognostic significance of MATR3 in stage I and II non-small cell lung cancer patients. J Cancer Res Clin Oncol 148, 3313–3322 (2022). https://doi.org/10.1007/s00432-022-04097-9
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DOI: https://doi.org/10.1007/s00432-022-04097-9