Abstract
Purpose
Basal cell carcinoma (BCC) is the most prevalent cancer worldwide. Different mechanisms are proposed to be involved in its pathogenesis such as oxidative stress. Oxidative stress, which is the consequence of the disruption of redox balance in favor of oxidants, is involved in the initiation or progression of many tumors. Thioredoxin reductase (TrxR) is a key enzyme of the thioredoxin (Trx) system, containing Trx and TrxR and NADPH, which is one of the main cellular oxidoreductases with an essential role in cellular health and survival through providing and maintaining redox balance. Therefore, we aimed to study and compare the activity and tissue distribution of TrxR in tumoral tissue and its healthy margin in patients with BCC.
Methods
After biopsy and taking samples from 18 patients, TrxR activity was measured using a commercial kit and its tissue distribution was assessed immunohistochemically.
Results
Both the activity and tissue distribution of TrxR in tumoral tissues were significantly higher compared to their healthy margins. Regarding the tissue distribution, this significant increase in TrxR in tumoral tissues was documented based on both staining intensity and abundance of positive cells in immunohistochemistry.
Conclusions
Based on these results, it is concluded that TrxR is involved in the pathogenesis of BCC; however, more investigations are required to clarify whether this increase is a consequence of BCC or it is an initiating mechanism.
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Acknowledgments
We are very grateful to all patients who participated in this study. This work was based on the Research Project No. 901151, as the MD dissertation of Maryam Sobhani, financed by the Research Council of Mashhad University of Medical Sciences.
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This study was funded by Mashhad University of Medical Sciences.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards (Ethical code from Mashhad University of Medical Sciences: 901151).
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Sobhani, M., Taheri, AR., Jafarian, AH. et al. The activity and tissue distribution of thioredoxin reductase in basal cell carcinoma. J Cancer Res Clin Oncol 142, 2303–2307 (2016). https://doi.org/10.1007/s00432-016-2242-0
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DOI: https://doi.org/10.1007/s00432-016-2242-0