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Expression of Helios in gastric tumor cells predicts better survival in gastric cancer patients

  • Original Article – Clinical Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Helios belongs to Ikaros family, which plays an important role in the cell-fate decision and control cell proliferation; abnormal expressions in leukemia are associated with poor prognosis. In this study, we investigated the Helios expression between Helicobacter pylori infection and prognosis in gastric cancer patients.

Methods

A total of 67 gastric cancer patients who received partial or full gastrectomies were enrolled. Helios expression by immunohistochemistry and mRNA was investigated with the clinical stage, Helicobacter pylori infection, CD4 expression, FoxP3 expression and prognosis.

Results

From the immunohistochemistry stain, we found that the Helios was expressed in both cancer cell and tumor-infiltrated lymphocytes. The high expression of Helios in gastric tumor cells had a better median overall survival rate in gastric cancer patients (50.7 ± 3.2 vs. 34.1 ± 4.9 months; P = 0.015), Helicobacter pylori-infected patients (51.1 ± 3.5 vs. 30.4 ± 5.1 months; P = 0.007) and advanced gastric cancer patients (42.1 ± 5.5 vs. 23.2 ± 4.8 months; P = 0.043). From multivariate analysis, the Helios expression in gastric tumor cells was an independent factor to predict better survival in all gastric cancers (HR = 2.78; 95 % confidence interval [CI], 1.09–7.09; P = 0.032) and advanced gastric cancer patients (HR = 2.85; 95 % confidence interval [CI], 1.00–8.13; P = 0.03).

Conclusions

Higher expression of Helios in gastric tumor cells predicts better survival in gastric cancer patients, especially for Helicobacter pylori-infected and advanced-stage gastric cancer patients.

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Acknowledgments

This work was supported by Chang Gung Memorial Hospital, Chiayi (Taiwan, Republic of China). We thank the Laboratory Animal Center, Department of Medical Research, Chang Gung Memorial Hospital at Chiayi; Tissue Bank, Department of Medical Research, Chang Gung Memorial Hospital at Chiayi; the EVERBIO AutoTiss 1000 tissue microarrayer service provided by the Expensive Advanced Instrument Core Laboratory, Department of Medical Research and Development, Chang Gung Memorial Hospital at Chiayi to help us in the experiments and tissue collection.

Funding

This study was funded by Chang Gung Memorial Hospital, Chia-Yi, TW. The funding number is CORPG6D0011, CORPG6D0012, CORPG6D0013, CORPG6D0021, CORPG6D0022, CORPG6D0023.

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Authors and Affiliations

  1. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chang Gung Memorial Hospital, Chiayi, Taiwan

    Wei-Ming Chen, Cheng-Shyong Wu & Hao-Chun Huang

  2. College of Medicine, Chang Gung University, Taoyuan, Taiwan

    Wei-Ming Chen & Cheng-Shyong Wu

  3. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan

    Wei-Ming Chen, Jing-Lan Liu & Pey-Jium Chang

  4. Department of Pathology, Chang Gung Memorial Hospital, Chiayi, Taiwan

    Jing-Lan Liu

  5. Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, No.168, University Rd., Min-Hsiung, Chia-Yi, 62247, Taiwan, Republic of China

    Chia-Ming Yeh, Libby Tseng & Shu-Fen Wu

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  1. Wei-Ming Chen
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Correspondence to Shu-Fen Wu.

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The authors declare that they have no conflict of interest.

Human and animal rights statement

The Institutional Review Board of the Chang Gung Memorial Hospital approved all human subject assessments (IRB No. 104-4187C).

Informed consent

The pathologic examination and further investigation were carried out under the informed consent of the patients.

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Chen, WM., Wu, CS., Liu, JL. et al. Expression of Helios in gastric tumor cells predicts better survival in gastric cancer patients. J Cancer Res Clin Oncol 142, 2375–2382 (2016). https://doi.org/10.1007/s00432-016-2223-3

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  • DOI: https://doi.org/10.1007/s00432-016-2223-3

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