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Journal of Cancer Research and Clinical Oncology

, Volume 142, Issue 10, pp 2209–2216 | Cite as

MassARRAY, pyrosequencing, and PNA clamping for EGFR mutation detection in lung cancer tissue and cytological samples: a multicenter study

  • Kyueng-Whan Min
  • Wan-Seop KimEmail author
  • Se Jin Jang
  • Yoo Duk Choi
  • Sunhee Chang
  • Soon Hee Jung
  • Lucia Kim
  • Mee-Sook Roh
  • Choong Sik Lee
  • Jung Weon Shim
  • Mi Jin Kim
  • Geon Kook Lee
  • Korean Cardiopulmonary Pathology Study Group
Original Article – Clinical Oncology

Abstract

Background

Testing for epidermal growth factor receptor (EGFR) mutation is an important process in the therapeutic plan of patients with lung cancer. Recently, MassARRAY, based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, has been shown to be a useful method for somatic mutation analysis with pyrosequencing and peptide nucleic acid clamping (PNAc).

Methods

A total of 107 tissues and 67 cytological samples, which were confirmed to have lung adenocarcinoma at nine hospitals in Korea, were collected. Among the MassARRAY, pyrosequencing, and PNAc, the concordance rates and sensitivity of EGFR mutation detection were analyzed and validated in comparative tissue and cytological specimens.

Results

The concordance rate between pyrosequencing and PNAc was higher than that between MassARRAY and either of the pyrosequencing and PNAc in both tissue and cytological samples. In a comparison of diagnostic performance, MassARRAY (sensitivity: 85.7 %) was higher than pyrosequencing (74.3 %) and PNAc (70 %) in tissue, although pyrosequencing (80.5 %) was more highly sensitive, compared to MassARRAY (70.7 %) and PNAc (70.7 %) in terms of cytology. Unexpectedly, use of MassARRAY resulted in a significantly different EGFR mutation detection rate between tissue and cytological samples.

Conclusions

When used for the detection of EGFR mutations, MassARRAY was more sensitive than pyrosequencing or PNA clamping in tissue, but not in cytological samples. In EGFR mutation detection between tissues and cytology, PNAc showed relatively higher concordance than MassARRAY or pyrosequencing.

Keywords

EGFR mutation Mass spectrometry, pyrosequencing Peptide nucleic acid Lung adenocarcinoma 

Notes

Acknowledgments

The authors thank all members of the Korean Cardio-Pulmonary Pathology Study Group for their support and their excellent input.

Funding

This research was conducted with the support of AstraZeneca (ISSIRES0079).

Compliance with ethical standards

Conflict of interest

There is no actual or potential conflict of interest to declare.

Ethical standard

This study was conducted under the approval of the Institutional Review Board of the Konkuk University Medical Center (KUH 1210016).

Supplementary material

432_2016_2211_MOESM1_ESM.docx (43 kb)
Supplementary material 1 (DOCX 42 kb)
432_2016_2211_MOESM2_ESM.docx (42 kb)
Supplementary material 2 (DOCX 41 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Kyueng-Whan Min
    • 1
  • Wan-Seop Kim
    • 2
    Email author
  • Se Jin Jang
    • 3
  • Yoo Duk Choi
    • 4
  • Sunhee Chang
    • 5
  • Soon Hee Jung
    • 6
  • Lucia Kim
    • 7
  • Mee-Sook Roh
    • 8
  • Choong Sik Lee
    • 9
  • Jung Weon Shim
    • 10
  • Mi Jin Kim
    • 11
  • Geon Kook Lee
    • 12
  • Korean Cardiopulmonary Pathology Study Group
  1. 1.Department of PathologyHanyang University Guri Hospital, Hanyang University College of MedicineGuri-siSouth Korea
  2. 2.Department of PathologyKonkuk University Medical Center, Konkuk University School of MedicineSeoulSouth Korea
  3. 3.Department of PathologyAsan Medical Center, University of Ulsan College of MedicineSeoulSouth Korea
  4. 4.Department of PathologyChonnam National University Hospital, Chonnam National University Medical SchoolGwangjuSouth Korea
  5. 5.Department of PathologyInje Unversity Ilsan Paik Hospital, Inje University School of MedicineGoyang-siSouth Korea
  6. 6.Department of PathologyWonju Severance Christian Hospital, Yonsei University Wonju College of MedicineWonju-siSouth Korea
  7. 7.Department of PathologyInha University Hospital, Inha University School of MedicineIncheonSouth Korea
  8. 8.Department of PathologyDong-A University Hospital, Dong-A University College of MedicineBusanSouth Korea
  9. 9.Department of PathologyChungnam National University Hospital, Chungnam National University College of MedicineDaejeonSouth Korea
  10. 10.Department of PathologyHallym University Dongtan Sacred Heart Hospital, Hallym University College of MedicineHwaseong-siSouth Korea
  11. 11.Department of PathologyYeungnam University Medical Center, Yeungnam University College of MedicineDaeguSouth Korea
  12. 12.Department of PathologyNational Cancer CenterGoyang-siSouth Korea

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