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High-risk and low-risk gastric cancer areas in Italy and its association with microsatellite instability

  • Original Article – Clinical Oncology
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Abstract

Purpose

The different pathological characteristics and prognoses between gastric cancer patients coming from high-risk (group A) and low-risk (group B) areas of Italy were analyzed. We investigated a suspected difference in microsatellite instability (MSI) between these two groups.

Methods

MSI analyses of 452 gastric cancer patients were performed using five quasimonomorphic mononucleotide repeats NR-21, NR-24, NR-27, BAT-25, and BAT-26. MSI analysis was done by PCR usage. An allelic profile of these five mononucleotides was detected on an automated DNA sequencer ABI PRISM 3100 Genetic Analyser. Data were analyzed according to high-risk and low-risk gastric cancer areas.

Results

MSI was observed in 23.9 % of all gastric cancer patients studied. Patients from group A showed a higher rate of MSI (28.4 %) than from group B (13.5 %) (p < 0.001). We analyzed this association together with tumor location and Lauren classification: A nonsignificant differences were seen when analyzing cardia and non-cardia tumors (p = 0.854) but significant for Lauren histotype (p = 0.028). There was no statistical difference in survival between high-risk and low-risk areas (p = 0.437), with a nonsignificant trend for better survival in the high-risk group, especially when measured over a longer period of time. Analyzing MSI or MSS in these groups, the survival curves were almost the same.

Conclusions

A higher frequency of MSI in patients coming from high-risk areas may help explain geographical differences in gastric cancer. The trend of better survival in high-risk areas may be due to a higher rate of MSI gastric cancer patients.

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Acknowledgments

This study was funded by Istituto Toscano Tumori (ITT) (Grant No. ITT-2007).

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Authors and Affiliations

  1. General Surgery and Surgical Oncology Department, University of Siena, viale Bracci 16, 53-100, Siena, Italy

    Karol Polom, Daniele Marrelli, Valeria Pascale, Costantino Voglino & Franco Roviello

  2. Section of Pharmacology and University Center DIFF-Drug Innovation Forward Future, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy

    Giandomenico Roviello

  3. University of Medical Sciences, Poznan, Poland

    Henry Rho

  4. Department of Pathology, Azienda Ospedaliera Universitaria Senese, Siena, Italy

    Carla Vindigni

  5. Department of Medicine, Surgery and Neurosciences, Unit of Endoscopy and Gastroenterology, University of Siena, Siena, Italy

    Mario Marini & Raffaele Macchiarelli

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  1. Karol Polom
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  2. Daniele Marrelli
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  3. Valeria Pascale
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  4. Giandomenico Roviello
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Correspondence to Karol Polom.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Polom, K., Marrelli, D., Pascale, V. et al. High-risk and low-risk gastric cancer areas in Italy and its association with microsatellite instability. J Cancer Res Clin Oncol 142, 1817–1824 (2016). https://doi.org/10.1007/s00432-016-2181-9

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  • DOI: https://doi.org/10.1007/s00432-016-2181-9

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