Abstract
Purpose
Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.
Methods
The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.
Results
Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.
Conclusions
Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (No. 81201637 and No. 81272207), and Jiehui Di was sponsored by Qing Lan Project of Jiangsu Province.
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We declare that we have no conflict of interest.
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Jiehui Di, Hui Huang, and Yan Wang have contributed equally to this work.
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Di, J., Huang, H., Wang, Y. et al. p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading. J Cancer Res Clin Oncol 141, 1791–1798 (2015). https://doi.org/10.1007/s00432-015-1948-8
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DOI: https://doi.org/10.1007/s00432-015-1948-8