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Prognostic significance of SERPINE2 in gastric cancer and its biological function in SGC7901 cells

Journal of Cancer Research and Clinical Oncology volume 141, pages 805–812 (2015)Cite this article

Abstract

Purpose

Altered expression of serine protease inhibitor peptidase inhibitor clade E member 2 (SERPINE2) associates with human cancer development and progression; thus, this study investigated SERPINE2 expression in gastric cancer tissues for association with clinicopathological and survival data from the patients and then investigated the role of SERPINE2 in gastric cancer cells in vitro.

Methods

The levels of SERPINE2 mRNA in 243 gastric cancer tissues and paired non-cancerous mucosa were determined using quantitative PCR. Inhibition of SERPINE2 expression by small interfering RNA (siRNA) was detected by Western blotting. tetrazolium, soft agar, and transwell assays were performed to evaluate the proliferation, anchorage-independent growth, and motility of gastric cancer SGC7901 cells transfected with SERPINE2 siRNA.

Results

Compared with the normal mucosa, SERPINE2 mRNA was increased in gastric cancer tissues and cells. Analysis of the 243 matched specimens showed that high SERPINE2 levels were associated with lymph node metastasis, distant metastasis, and clinical stage. Patients with high SERPINE2 mRNA levels had poorer survival compared with patients with low SERPINE2 mRNA levels. In vitro, SERPINE2 inhibited anchorage-independent growth, migration, and invasion of SGC7901 cells, but not proliferation.

Conclusions

Our findings indicate that upregulated SERPINE2 may contribute to the aggressive phenotype of gastric cancer and suggest that SERPINE2 can be used as a novel prognostic factor and anticancer target in patients with gastric cancer.

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Acknowledgments

This study is supported by Shandong Province Health Science and Technology Development Program (2009HZ067).

Conflict of interest

All authors promise that there is no conflict to disclose.

Author information

Affiliations

  1. Department of General Surgery, Qianfoshan Hospital, Shandong University, 16766 Jing Shi Road, Jinan, 250014, China

    Kun Wang, Bin Wang, Guang Xin Li & Zhen Hai Yu

  2. Department of Pathology, School of Medicine, Shandong University, 44 Wen Hua Xi Road, Jinan, 250012, China

    Ai Yan Xing

  3. Department of Pathology, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan, 250012, China

    Ai Yan Xing

  4. Department of General Surgery, Qilu Hospital, Shandong University, 107 Wen Hua Xi Road, Jinan, 250012, China

    Ke Sen Xu

Authors
  1. Kun Wang
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  2. Bin Wang
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  3. Ai Yan Xing
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  4. Ke Sen Xu
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  5. Guang Xin Li
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  6. Zhen Hai Yu
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Corresponding authors

Correspondence to Guang Xin Li or Zhen Hai Yu.

Additional information

Kun Wang and Bin Wang have contributed equally to this article.

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Wang, K., Wang, B., Xing, A.Y. et al. Prognostic significance of SERPINE2 in gastric cancer and its biological function in SGC7901 cells. J Cancer Res Clin Oncol 141, 805–812 (2015). https://doi.org/10.1007/s00432-014-1858-1

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  • DOI: https://doi.org/10.1007/s00432-014-1858-1

Keywords

  • SERPINE2
  • Gastric cancer
  • Prognosis
  • Migration
  • Invasion
  • Growth
  • Tumor aggressiveness