Abstract
Background
Gastric cancer (GC) is one of the most common malignancies in China. B-cell translocation gene 3 (BTG3) has been identified as a tumor suppressor in several tumors, but its role in GC remains unknown. This study aimed to detect the expression of BTG3 and its prognostic value in GC tissues and determine its function in the progression of GC.
Methodology
The expression of BTG3 was detected in GC cell lines and tissues by real-time RT-PCR, Western blot or immunohistochemistry. A series of in vitro and in vivo assays were performed to evaluate the effect of BTG3 on proliferation, migration and invasion of GC cells.
Results
B-cell translocation gene 3 was obviously down-regulated in GC tissues. Its expression was positively correlated with distant metastasis (P < 0.05). Patients with lower BTG3 expression had shorter overall survival time (P = 0.015). BTG3 suppressed the proliferation of GC cells in vitro and in vivo. It also inhibited migration and invasion of GC cells in vitro.
Conclusion
Down-regulation of BTG3 is closely associated with proliferation, migration and invasion in GC. It may be a novel prognostic biomarker for GC patients.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (81272759, 81172382); Natural Science Foundation of Guangdong Province (S2012010009669, S2013010014544); Research Fund for the Science and technology Star of Zhujiang City of Guangzhou Province (2011J2200074). We thank for Professor Reddy for editing the English writing.
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We declare that we have no conflict of interest.
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X. L. Ren and X. H. Zhu have contributed equally to this work.
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Ren, X.L., Zhu, X.H., Li, X.M. et al. Down-regulation of BTG3 promotes cell proliferation, migration and invasion and predicts survival in gastric cancer. J Cancer Res Clin Oncol 141, 397–405 (2015). https://doi.org/10.1007/s00432-014-1826-9
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DOI: https://doi.org/10.1007/s00432-014-1826-9