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Elevated circulating levels of tissue factor-positive microvesicles are associated with distant metastasis in lung cancer

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Abstract

Purpose

Microvesicles (MV) in the blood stream are associated with distant metastasis in cancer. Platelet or endothelial cell-related MV actively participate in thrombogenesis, which is an important step in cancer metastasis. This study investigated the correlations between MV levels of platelet-poor plasma and distant metastasis in lung cancer.

Methods

Platelet-poor plasma from 44 treatment-naive lung cancer (23 with distant metastasis) and 19 normal subjects was used to determine the levels of glycoprotein Iβ (CD42) + platelet MV (PMV), P-selectin (CD62P) + PMV, VE-cadherin (CD144) + endothelial MV (EMV), tissue factor (CD142) + MV, thrombin-antithrombin complex and vascular endothelial growth factor (VEGF).

Results

The level of CD142 + MV was significant (odds ratio 5.86, 95 % confidence interval 1.31–38.3) in predicting distant metastasis in lung cancer, and a cutoff value of 2.668 (after logarithm transformation) in the ROC curve had a specificity of 90 % and a sensitivity of 59 %. The presence of distant metastasis showed a significant correlation between CD144 + EMV and VEGF, but not between CD144 + EMV and CD42 + PMV or CD62P + PMV in lung cancer subjects.

Conclusions

The finding of CD142 + MV in platelet-poor plasma may be useful for suggesting distant metastasis in lung cancer. In addition to thrombogenesis, interaction between VE-cadherin and VEGF may be needed for successful metastasis in lung cancer.

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Acknowledgments

This study was supported by a grant from the Chang Gung Memorial Hospital at Keelung (CMRPG260591).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Internal Medicine, Chang Gung Memory Hospital, Keelung, Taiwan, ROC

    Jo-Chi Tseng, Yu-Chih Liu, Hung-Jie Chen & Chung-Ching Hua

  2. Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan, ROC

    Jo-Chi Tseng

  3. Department of Emergency Medicine, Chang Gung Memory Hospital, Linkou, Taiwan, ROC

    Jo-Chi Tseng

  4. Department of Pathology, Chang Gung Memory Hospital, Keelung, Taiwan, ROC

    Liang-Che Chang

  5. Department of Internal Medicine, Cardinal Tien Hospital, Yonghe Branch, Taiwan, ROC

    Boy-Yiing Jiang

  6. Department of Pulmonary Medicine, Chang Gung Memorial Hospital, Linkou, Taiwan, ROC

    Chih-Teng Yu

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  1. Jo-Chi Tseng
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Correspondence to Chung-Ching Hua.

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Tseng, JC., Chang, LC., Jiang, BY. et al. Elevated circulating levels of tissue factor-positive microvesicles are associated with distant metastasis in lung cancer. J Cancer Res Clin Oncol 140, 61–67 (2014). https://doi.org/10.1007/s00432-013-1544-8

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  • DOI: https://doi.org/10.1007/s00432-013-1544-8

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