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Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population

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Abstract

Introduction

Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be associated with the risk of colorectal cancer (CRC).

Materials and methods

We genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay.

Results

The rs11614913 CT, TT genotypes, and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR = 7.34, 95 % CI 3.76–14.34; TT vs. CC: OR = 13.66, 95 % CI 6.76–27.6; T vs. C: OR = 1.99, 95 % CI 1.63–2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR = 1.49, 95 % CI 1.02–2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR = 0.58, 95 % CI 0.37–0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR = 0.80, 95 % CI 0.66–0.97, p = 0.02).

Conclusion

These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC.

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Acknowledgments

This study was financially supported by the Natural Science Foundation of China (Grant No:81000515) and New Teachers’ Fund for Doctor Stations, Ministry of Education of China (Grant No : 20090181120021).

Conflict of interest

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Authors and Affiliations

  1. Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Meili Lv & Wei Dong

  2. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Lijuan Li, Lushun Zhang, Xiaowei Su, Li Wang & Lin Zhang

  3. Laboratory of Molecular Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, People’s Republic of China

    Linbo Gao & Lin Zhang

  4. Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu, 610041, Sichuan, People’s Republic of China

    Linbo Gao & Lin Zhang

Authors
  1. Meili Lv
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  2. Wei Dong
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  3. Lijuan Li
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  4. Lushun Zhang
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  5. Xiaowei Su
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  6. Li Wang
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  7. Linbo Gao
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  8. Lin Zhang
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Corresponding authors

Correspondence to Linbo Gao or Lin Zhang.

Additional information

Meili Lv and Wei Dong contributed equally to this work.

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Lv, M., Dong, W., Li, L. et al. Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population. J Cancer Res Clin Oncol 139, 1405–1410 (2013). https://doi.org/10.1007/s00432-013-1456-7

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  • DOI: https://doi.org/10.1007/s00432-013-1456-7

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