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Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines by proteome analysis

Journal of Cancer Research and Clinical Oncology volume 139, pages 509–519 (2013)Cite this article

Abstract

Purpose

Resistance to chemotherapy drugs remains a difficult problem in bladder cancer treatment. Protein expression is an important factor underlying multidrug resistance (MDR) in bladder cancer. The aim of the study was to explore differentially expressed proteins responsible for MDR between an adriamycin-resistant human bladder cancer cell line (pumc-91/ADM) and its parental cell line (pumc-91).

Methods

Two-dimensional gel electrophoresis (2-DE) combining image analysis was used to screen the differentially expressed protein spots between the pumc-91/ADM and pumc-91 cell lines. Then, the protein spots were identified using MALDI-TOF/TOF mass spectrometry. Among the identified proteins, annexin A2 (ANXA2) and nucleophosmin (NPM1) were then further verified using RT-PCR and Western blot analysis.

Results

A total of 30 proteins, including 19 up-regulated and 11 down-regulated proteins, were successfully identified in pumc-91/ADM. According to their different functions, these 30 proteins were classified into 12 categories. Annexin A2 (ANXA2) and nucleophosmin (NPM1) were up-regulated in pumc-91/ADM compared with pumc-91.

Conclusion

The proteins identified may have an important clinical significance in MDR, and ANXA2 and NPM1 may take part in mechanism of MDR in bladder cancer.

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Acknowledgments

Research supported by Beijing Municipal Natural Science Foundation.

Conflict of interest

We declare that we have no conflict of interest.

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Affiliations

  1. Department of Clinical Laboratory Center, Shijitan Hospital Affiliated with Capital Medical University, Beijing, China

    Qian Meng, Ting Lei, Min Zhang, Jin Zhao, Xu-Hong Zhao & Man Zhang

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  1. Qian Meng
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  2. Ting Lei
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  3. Min Zhang
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  4. Jin Zhao
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  5. Xu-Hong Zhao
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  6. Man Zhang
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Correspondence to Man Zhang.

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Meng, Q., Lei, T., Zhang, M. et al. Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines by proteome analysis. J Cancer Res Clin Oncol 139, 509–519 (2013). https://doi.org/10.1007/s00432-012-1350-8

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  • DOI: https://doi.org/10.1007/s00432-012-1350-8

Keywords

  • Bladder cancer
  • Multidrug resistance
  • Annexin A2
  • Nucleophosmin