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UbcH10 expression provides a useful tool for the prognosis and treatment of non-small cell lung cancer

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Abstract

Purpose

To investigate the expression pattern of UbcH10 in non-small cell lung cancer (NSCLC) and its correlations with clinicopathological features and prognostic value in NSCLC patients.

Methods

The UbcH10 expression in NSCLC tissues, SK-MES-1, and A549 lung cancer cell lines was evaluated, and its correlation with clinicopathological features and prognostic value in NSCLC patients was examined by Kaplan–Meier analysis and Cox regression analysis. The biological effects of UbcH10 on the cell proliferation, cell cycle, and chemosensitivity to gemcitabine or paclitaxel in SK-MES-1 cells were examined upon the UbcH10 silencing.

Results

UbcH10 is overexpressed in poorly differentiated NSCLC samples than in well-differentiated ones, and its expression level was significantly higher in squamous cell carcinoma than that in adenocarcinoma. Higher UbcH10 expression was associated with a shorter postoperative survival time of NSCLC patients by Kaplan–Meier method and was found to be an independent risk factor that influences the postoperative survival time of NSCLC patients by Cox regression analysis. UbcH10 mRNA and protein expression were significantly upregulated in SK-MES-1 cells compared with A549 and MRC-5 cells. Suppression of UbcH10 expression in SK-MES-1 cells inhibited cell proliferation and increased chemosensitivity to gemcitabine or paclitaxel concomitant with decreased MDR1 gene expression.

Conclusion

UbcH10 may play an important role in NSCLC carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC.

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Abbreviations

UbcH10:

Ubiquitin-conjugating enzyme E2C

NSCLC:

Non-small cell lung cancer

SCC:

Squamous cell carcinoma

AC:

Adenocarcinomas

P-D:

Poorly differentiated

M-D:

Moderately differentiated

W-D:

Well differentiated

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Acknowledgments

This work was supported by the National Natural Sciences Fund Project of China (NSFC nos. 30901540).

Conflict of interest

All authors do not have any possible conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Respiratory Disease, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, China

    Liming Zhao, Jiquan Chen, Qingyu Xiu & Bing Li

  2. Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Lei Jiang

  3. Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Liangzhe Wang, Jin He & Hongyu Yu

  4. Department of Thoracic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China

    Guangyuan Sun

Authors
  1. Liming Zhao
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  2. Lei Jiang
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  3. Liangzhe Wang
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  4. Jin He
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  5. Hongyu Yu
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  6. Guangyuan Sun
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  7. Jiquan Chen
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  8. Qingyu Xiu
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  9. Bing Li
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Corresponding author

Correspondence to Bing Li.

Additional information

Liming Zhao and Lei Jiang contributed equally to this work.

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Zhao, L., Jiang, L., Wang, L. et al. UbcH10 expression provides a useful tool for the prognosis and treatment of non-small cell lung cancer. J Cancer Res Clin Oncol 138, 1951–1961 (2012). https://doi.org/10.1007/s00432-012-1275-2

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  • DOI: https://doi.org/10.1007/s00432-012-1275-2

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