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GDF3 inhibits the growth of breast cancer cells and promotes the apoptosis induced by Taxol

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Abstract

Purpose

The aim of this study is to investigate whether GDF3 is related to the progression of human breast cancer and the effects of GDF3 on breast cancer cells.

Methods

The expression of GDF3 in 24 breast cancer specimens paired with corresponding neighboring nontumorous tissue was studied by Western blot. Breast cancer cells were treated with different concentrations of recombinant human GDF3 protein. Using lentivirus containing sh-RNA, we knocked down the expression of GDF3. Soft agar assay was performed to explore the effects of GDF3 on colony formation. Different anti-tumor drugs dealt with MCF-7 cells stably expressing GDF3.

Results

We found that GDF3 expression level was significantly down-regulated in breast cancer tissues compared to the surrounding nontumorous tissues. GDF3 proteins could inhibit the proliferation of MCF-7 and T47D cells. We also found that the knockdown of GDF3 resulted in the promotion of colony formation and enhanced the ability of anchorage-independent cell growth in soft agar. Furthermore, overexpression of GDF3 could promote the apoptosis induced by Taxol.

Conclusions

Our data indicated that GDF3 expression is significantly decreased in human breast cancer tissues, and reconstitution of GDF3 in breast cancer may be a potential therapeutic approach to inhibit aggressive growth of breast cancer.

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Acknowledgments

We thank Dr. Zhimin Shao and Dr. Zhouluo Ou (Department of Medical Oncology, Cancer Hospital of Fudan University, Shanghai Medical College, Shanghai, PR China) for their kindly help in cancer specimens collecting. This work was supported by the National 863 project of China (2006AA020501), the National Key Sci-Tech Special Project of China (2008ZX10002-020), the Project of the Shanghai Municipal Science and Technology Commission (03dz14086).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China

    Qiang Li, Yichen Ling & Long Yu

  2. Division of Molecular Medicine, Cardiovascular Research Laboratories, Department of Anesthesiology and Medicine, David Geffen School of Medicine at University of California Los Angeles (UCLA), Los Angeles, CA, USA

    Qiang Li

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  1. Qiang Li
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  2. Yichen Ling
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Correspondence to Long Yu.

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Li, Q., Ling, Y. & Yu, L. GDF3 inhibits the growth of breast cancer cells and promotes the apoptosis induced by Taxol. J Cancer Res Clin Oncol 138, 1073–1079 (2012). https://doi.org/10.1007/s00432-012-1213-3

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  • DOI: https://doi.org/10.1007/s00432-012-1213-3

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