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Effect of shRNA targeting survivin on ovarian cancer

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Abstract

Purpose

This study investigated the effect of shRNA targeting survivin on cultured ovarian cancer cells and on a murine ovarian cancer xenograft.

Methods

An RNAi plasmid for survivin was transfected into SKOV3 cells, and the effect of shRNA targeting survivin on the expression of survivin was determined. Transmission electron microscopy (TEM), flow cytometry, and TUNEL staining were used to assess apoptosis. The MTT assay was used to measure cell growth and changes in cisplatin sensitivity. SKOV3 cells were injected into nude mice, and the effect of shRNA targeting the survivin gene on tumor growth was assessed.

Results

SKOV3 cells transfected with an RNAi plasmid against survivin had increased apoptosis and slower growth. At the molecular level, these cells also had lower expression of survivin. Nude mice inoculated with SKOV3 cells developed cancers, and treatment with shRNA targeting survivin markedly inhibited the growth of these cancers with no obvious side effects.

Conclusions

Our studies of SKOV3 cells and ovarian cancer xenografts in nude mice indicate that shRNA targeting survivin has potential for the treatment of ovarian cancer.

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, The Third Hospital of Harbin Medical University, No. 150, Ha Ping Road, Nan Gang District, Heilongjiang Province, People’s Republic of China

    Jun Xing

  2. Department of Obstetrics, The Fourth Hospital of Harbin Medical University, No. 37, Yi Yuan Avenue, Nan Gang District, Heilongjiang Province, People’s Republic of China

    Chang-Ru Jia & Yan Cai

  3. Department of Internal Medicine, The Third Hospital of Harbin Medical University, No. 150, Ha Ping Road, Nan Gang District, Heilongjiang Province, People’s Republic of China

    Yan Wang

  4. Department of Obstetrics and Gynecology, Jian Hua Hospital, Jian Hua District, Qiqihar Province, People’s Republic of China

    Jing Guo

Authors
  1. Jun Xing
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  2. Chang-Ru Jia
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  3. Yan Wang
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  4. Jing Guo
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  5. Yan Cai
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Corresponding author

Correspondence to Yan Wang.

Additional information

Jun Xing and Chang-Ru Jia had equal contribution to this paper.

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Xing, J., Jia, CR., Wang, Y. et al. Effect of shRNA targeting survivin on ovarian cancer. J Cancer Res Clin Oncol 138, 1221–1229 (2012). https://doi.org/10.1007/s00432-012-1196-0

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  • DOI: https://doi.org/10.1007/s00432-012-1196-0

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