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The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma

Journal of Cancer Research and Clinical Oncology volume 138, pages 49–56 (2012)Cite this article

Abstract

Purpose

Dysregulation of microRNA (miRNA) metabolism has been observed in a variety of human cancers, but the expression patterns of the enzymes responsible for generating miRNAs remain largely unexplored. In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.

Methods

Dicer and Drosha mRNA levels were detected by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) using 24 NPC tissues, 7 normal nasopharyngeal epithelium samples (NPE) and NPC cell lines. In addition, protein levels were detected by immunohistochemistry (IHC) using an NPC tissue microarray (TMA), which include 251 NPC and 105 NPE cases. For some NPC patients can not be contacted, the survival data were available only for 146 patients. Kaplan–Meier analysis was performed, and the chi-square and log-rank tests were used to detect significance levels using SPSS 15.0 software.

Results

The mean level of Dicer and Drosha mRNA were significantly down-regulated in NPC tissue specimens and cell lines when compared with controls. The low levels of Dicer and Drosha protein were frequently seen in NPC, and the low expression of Dicer and Drosha protein was significantly correlated with shorter progression-free survival (PFS) and overall survival (OS) of NPC patients.

Conclusions

We observed that Drosha and Dicer expression was dysregulation in NPC compared with healthy control samples and was significantly correlated with shorter PFS and OS of NPC patients. Therefore, we hypothesise that the expression levels of Dicer and Drosha could be used as potential prognostic biomarkers for NPC.

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Acknowledgments

We are grateful to the Ear, Nose and Throat (ENT) Department at Xiangya Hospital for providing NPC samples. Guiyuan Li has received the 111 project (111-2-12) research grants. Wenling Zhang has received the National Science Foundation of China (81071644). Wei Xiong has received the Program for New Century Excellent Talents in University (NCET-08-0562).

Conflict of interest

We declare that we have no financial and personal relationships with other people or organisations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the manuscript entitled “The microRNA-Processing Enzymes Drosha and Dicer can Predict Prognosis of Nasopharyngeal Carcinoma”.

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Affiliations

  1. Cancer Research Institute, Central South University, 110 Xiang-Ya Road, Changsha, 410078, Hunan, People’s Republic of China

    Xiaofang Guo, Qianjin Liao, Pan Chen, Wei Xiong, Jian Ma, Xiaoling Li, Zhaohui Luo, Hailin Tang, Min Deng, Yin Zheng, Rong Wang, Wenling Zhang & Guiyuan Li

  2. Changsha Medical University, 9 Kilometers’ Stop at Leifeng Road, Yuelu District, Changsha, 10219, Hunan, People’s Republic of China

    Xiaofang Guo

  3. Cancer Research Institute, University of South China, 28 Chang-sheng West Road, Hengyang, 421001, Hunan, People’s Republic of China

    Qianjin Liao, Hailin Tang & Min Deng

  4. The Third Xiangya Hospital, Central South University, Changsha, 410078, Hunan, People’s Republic of China

    Xiayu Li

Authors
  1. Xiaofang Guo
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  2. Qianjin Liao
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  3. Pan Chen
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  4. Xiayu Li
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  5. Wei Xiong
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  6. Jian Ma
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  7. Xiaoling Li
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  8. Zhaohui Luo
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  9. Hailin Tang
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  10. Min Deng
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  11. Yin Zheng
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  12. Rong Wang
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  13. Wenling Zhang
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  14. Guiyuan Li
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Corresponding authors

Correspondence to Wenling Zhang or Guiyuan Li.

Additional information

Xiaofang Guo and Qianjin Liao contributed equally to this work.

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Guo, X., Liao, Q., Chen, P. et al. The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma. J Cancer Res Clin Oncol 138, 49–56 (2012). https://doi.org/10.1007/s00432-011-1058-1

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  • DOI: https://doi.org/10.1007/s00432-011-1058-1

Keywords

  • Dicer
  • Drosha
  • Nasopharyngeal carcinoma
  • Progression-free survival
  • Overall survival