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Prognostic and metastatic value of phosphatase of regenerating liver-3 in invasive breast cancer

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Abstract

Purpose

The aim of this study was to investigate the expression of the PRL-3 in human invasive breast cancer and to evaluate its clinical and prognostic significance. Its potential role in the invasive-metastatic properties of invasive breast cancer was also investigated.

Methods

Protein expression of PRL-3 was evaluated by immunohistochemistry for a consecutive series of 82 invasive human breast cancer tissues and 63 matched lymph node metastases, including PRL-3 mRNA expression analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) in malignant, nonmalignant breast tissue samples and lymph node metastases. We investigated the correlation of PRL-3 with clinicopathologic features, Overall and recurrence-free survival distribution curves were assessed using the Kaplan–Meier test and log-rank statistics, followed by Cox proportional hazards regression model.

Results

We found that 70.7% patients expressed a high level of PRL-3 protein in their tumors, and its over expression was positive correlated with lymph node metastasis (LNM) (P = 0.011). Moreover, The PRL-3 mRNA expression was significantly higher in malignant compared to benign breast tissue, while increased expression of PRL-3 mRNA was significantly associated with LNM (P = 0.002). Univariate analysis showed that the positive expression of PRL-3 was a poor risk prognostic factor (OS, P = 0.045; RFS, P = 0.034). Multivariate analysis using the Cox regression model indicated that high PRL-3 expression was an independent unfavorable prognostic factor for RFS.

Conclusions

These results strongly suggest that PRL-3 expression can indicate the potential role of LNM to some extent. Increasing the risk of tumor metastasis (OR = 3.889). Our results also imply that PRL-3 might be a novel molecular marker for predicting relapse of invasive breast cancer.

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Abbreviations

PRL-3:

Phosphatase of regenerating liver-3

RT-PCR:

Reverse transcriptase-polymerase chain reaction

LNM:

Lymph node metastasis

OR:

Odds ratio

OS:

Overall survival

RFS:

Recurrence free survival

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Acknowledgments

The authors would like to thank Dr. Xiu-Ling Wu (Department of Pathology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou) for his valuable input, and Ms. Li Wan for her technical assistance.

Conflict of interest statement

The authors declare that no one has conflicts of interests with the study presented.

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Authors and Affiliations

  1. Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China

    Ru-Tian Hao, Xiao-Hua Zhang, Yi-Fei Pan, Hai-Guang Liu & You-Qun Xiang

  2. Department of Pathology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, 325000, China

    Li Wan & Xiu-Ling Wu

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  1. Ru-Tian Hao
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  2. Xiao-Hua Zhang
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  6. Li Wan
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Correspondence to Xiao-Hua Zhang.

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Hao, RT., Zhang, XH., Pan, YF. et al. Prognostic and metastatic value of phosphatase of regenerating liver-3 in invasive breast cancer. J Cancer Res Clin Oncol 136, 1349–1357 (2010). https://doi.org/10.1007/s00432-010-0786-y

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  • DOI: https://doi.org/10.1007/s00432-010-0786-y

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