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LAPTM4B-35 overexpression is a risk factor for tumor recurrence and poor prognosis in hepatocellular carcinoma

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Abstract

Purpose

Lysosomal protein transmembrane 4 beta-35 (LAPTM4B-35) is a tetra-transmembrane glycoprotein that is abundantly localized on membrane-bound organelles including endosomes and lysosomes, and promotes cell proliferation and tumorigenesis through regulation of cell cycle and signaling pathways. The aim of the present study is to determine the potential clinical implications of LAPTM4B-35 expression in hepatocellular carcinoma (HCC).

Methods

Immunohistochemistry assay was used to determine the expression of LAPTM4B-35 protein in normal and HCC tissues from 71 patients. The correlations of LAPTM4B-35 expression with clinicopathological parameters, including gender, age, background liver, viral status, tumor size, portal vein invasion, histopathological differentiation, serum AFP level, TNM staging and recurrence of HCC were assessed by Chi-squared test. Patient survival and their differences were determined by Kaplan–Meier method and log-rank test. Cox regression (Proportional hazard model) was adopted for multivariate analysis of prognostic factors.

Results

LAPTM4B-35 immunoreactivity was negative or low in normal liver tissues, but high in HCC tissues (51/71, 71.8%). The overexpression of LAPTM4B-35 was significantly associated with recurrence, TNM staging and portal vein invasion of HCC. Patients with high LAPTM4B-35 expression had significantly poorer overall survival (OS) and disease-free survival (DFS) (both P < 0.001) when compared with patients with the low expression of LAPTM4B-35. On multivariate analysis, LAPTM4B-35 expression was found to be an independent prognostic factor for OS and DFS (P = 0.018 and P = 0.001, respectively).

Conclusion

LAPTM4B-35 expression showed a strong association with the potencies of recurrence and metastasis and progression of HCC, and that may be applied as a novel marker for the prediction of recurrence and metastasis potency of HCC, and helpful for improving the diagnosis, prognosis and treatment of HCC.

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Authors and Affiliations

  1. Department of Cell Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China

    Hua Yang, Fu Xia Xiong, Ming Lin & Rou Li Zhou

  2. Center for Molecular Physiology Research, Children’s National Medical Center, Washington, DC, 20010, USA

    Yu Yang

  3. Department of Pathology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China

    Xiu Nie

Authors
  1. Hua Yang
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  2. Fu Xia Xiong
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  3. Ming Lin
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  4. Yu Yang
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  6. Rou Li Zhou
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Correspondence to Rou Li Zhou.

Additional information

Hua Yang and Fu Xia Xiong have contributed equally to this work

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Yang, H., Xiong, F.X., Lin, M. et al. LAPTM4B-35 overexpression is a risk factor for tumor recurrence and poor prognosis in hepatocellular carcinoma. J Cancer Res Clin Oncol 136, 275–281 (2010). https://doi.org/10.1007/s00432-009-0659-4

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  • DOI: https://doi.org/10.1007/s00432-009-0659-4

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