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Study on relationship between angiogenesis and micrometastases of peripheral blood in breast cancer

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Abstract

Purpose

To investigate the relationship between microvessel density, expression of vascular endothelial growth factor A (VEGF-A) and micrometastases in peripheral blood of patients with breast cancer.

Method

Microvessel density (MVD) and expression of VEGF-A were detected by immunohistochemistry S-P. Nested RT-PCR was introduced to detect the expression of hMAM mRNA in peripheral blood of all cases.

Result

Average MVD was 28.95 ± 6.95 microvessels/100× and positive rate of VEGF-A was 64.0% (32/50) in 50 cases with breast cancer. MVD count and expression of VEGF-A were related to tumor size, metastasis of axillary lymph nodes and clinical stages (P < 0.05), independent of age and histological classification (P > 0.05). The positive rate of hMAM mRNA in peripheral blood was 34.0% (17/50), which correlated with lymphatic metastasis and clinical stages (P < 0.05), independent of pathological category, menopause and hormone receptor (P > 0.05). MVD count and positive rate of VEGF-A in breast cancer with positive expression of hMAM mRNA was obviously higher than those without hMAM mRNA expression (χ 2 = 5.766, P = 0.032; t = 5.37, P = 0.002).

Conclusions

MVD count and positive expression of VEGF-A closely correlated to hMAM mRNA released from tumor cells in the circulation. hMAM mRNA is expected to become a valuable marker for further study on micrometastases of breast cancer.

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Acknowledgments

We thank Dr. Li Yan for providing MCF-7 cell line. We thank Prof. Liu Yongye for critical revision of the manuscript.

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Authors and Affiliations

  1. Oncology Department, General Hospital of Shenyang Military Region, 110016, Shenyang, Liaoning, China

    Xiao-Dong Xie, Shu-Xian Qu, Zhao-Zhe Liu, Fang Zhang & Zhen-Dong Zheng

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  1. Xiao-Dong Xie
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  2. Shu-Xian Qu
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  3. Zhao-Zhe Liu
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  4. Fang Zhang
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  5. Zhen-Dong Zheng
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Correspondence to Xiao-Dong Xie.

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Xie, XD., Qu, SX., Liu, ZZ. et al. Study on relationship between angiogenesis and micrometastases of peripheral blood in breast cancer. J Cancer Res Clin Oncol 135, 413–419 (2009). https://doi.org/10.1007/s00432-008-0473-4

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  • DOI: https://doi.org/10.1007/s00432-008-0473-4

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